Genome location: Pf3D7_05_v3:1,132,901..1,135,534(-)
Genome classification: Core
Product Description: ubiquitin carboxyl-terminal hydrolase 14
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0070628 | proteasome binding |
| Function | GO:0004843 | thiol-dependent deubiquitinase |
| Process | GO:0016579 | protein deubiquitination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.45 | 0.79 |
| Trophozoite | expressed | 1.15 | 0.43 |
| Schizont | expressed | 0.91 | 0.50 |
| Gametocyte | expressed | 0.43 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0527200
Resistome Missense Mutations: E515Q
Resistome Compounds with Missense Mutations: MMV006901
Resistome # Samples with Disruptive Mutations: 5 (5 missense, 5 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.179 | MFS: -2.737 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1242000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1242000)
Modification: Disrupted | RMgm-3375
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3J3
"Best" AlphaFill ligand hit: AYE (allylamine, Local RMSD=0.73) with 5L8W (Global RMSD=5.74)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.19.12 | ubiquitinyl hydrolase 1 | KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P54578 | Ubiquitin carboxyl-terminal hydrolase 14 | Homo sapiens | OMA, OrthoDB | US11242361, Compound 86US11242361, Compound 87US11242361, Compound 88US11242361, Compound 98... |
Ortholog Group (OrthoMCL): OG6_101892
Most Similar Human Ortholog: J3KS55
TM-align score: 0.74 | RMSD: 0.74
Seq Identity: 0.52 | Length: 27 / 605
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000101557 | ubiquitin specific peptidase 14 |
Protein Length: 605 | Molecular Weight (kDa): 70.733
UniProt ID(s): Q8I3J3
PDB ID(s): None
Isoelectric Point: 5.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000626 | Ubiquitin-like domain |
| InterPro | IPR001394 | Peptidase C19, ubiquitin carboxyl-terminal hydrolase |
| InterPro | IPR018200 | Ubiquitin specific protease, conserved site |
| InterPro | IPR029071 | Ubiquitin-like domain superfamily |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00240 | Ubiquitin-like domain |
| PFam | PF00443 | Peptidase C19, ubiquitin carboxyl-terminal hydrolase |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| Superfamily | SSF54236 | Ubiquitin-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 52 | 23 | 47 |
| disruptive | 34 | 94 | 41 | 95 |
| missense | 13 | 74 | 37 | 81 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 85 | 29 | 44 |
| disruptive | 73 | 172 | 71 | 151 |
| missense | 33 | 136 | 59 | 102 |
PlasmoDB Total SNPs: 137
Non-coding: 59 | Synonymous: 29 | Nonsynonymous: 49 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 26639022 | Characterization of the 26S proteasome network in Plasmodium falciparum. | Wang L, Delahunty C, ..., Becker K | 10.1038/srep17818 |