Genome location: Pf3D7_05_v3:1,135,566..1,138,779(-)
Genome classification: Core
Product Description: methionine aminopeptidase 1a, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.11.18 (Methionyl aminopeptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0070006 | metalloaminopeptidase activity |
| Function | GO:0008235 | metalloexopeptidase activity |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.12 | 0.93 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.09 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0527300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.857 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1242100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3J2
"Best" AlphaFill ligand hit: LMR (l-malate, Local RMSD=0.41) with 3TAV (Global RMSD=1.49)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.11.18 | methionyl aminopeptidase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q01662 | Methionine aminopeptidase 1 | Saccharomyces cerevisiae | OrthoDB | CHEMBL360513CHEMBL359541CHEMBL89671CHEMBL313650... |
| P53582 | Methionine aminopeptidase 1 | Homo sapiens | OrthoDB | bestatin-type inhibitor, 4bestatin-type inhibitor, 5bestatin-type inhibitor, 8CHEMBL327579... |
Protein Length: 327 | Molecular Weight (kDa): 37.411
UniProt ID(s): Q8I3J2
PDB ID(s): None
Isoelectric Point: 8.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000994 | Peptidase M24 |
| InterPro | IPR001714 | Peptidase M24, methionine aminopeptidase |
| InterPro | IPR002467 | Peptidase M24A, methionine aminopeptidase, subfamily 1 |
| InterPro | IPR036005 | Creatinase/aminopeptidase-like |
| PFam | PF00557 | Peptidase M24 |
| Superfamily | SSF55920 | Creatinase/aminopeptidase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 11 | 13 | 28 |
| disruptive | 1 | 18 | 15 | 52 |
| missense | 1 | 18 | 13 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 37 | 14 | 23 |
| disruptive | 4 | 65 | 33 | 90 |
| missense | 2 | 55 | 30 | 66 |
PlasmoDB Total SNPs: 187
Non-coding: 161 | Synonymous: 17 | Nonsynonymous: 7 | Stop Codon: 2
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |