PF3D7_0527900 (DDX41)

Genome location: Pf3D7_05_v3:1,153,516..1,156,002(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DDX41, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.71	0.67
Trophozoite	expressed	0.56	0.69
Schizont	expressed	0.10	0.94
Gametocyte	expressed	0.38	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0527900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.451 | MFS: -2.737 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1242500)

Relative Growth Rate: 0.17 ± 0.21
Confidence: 4.46

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8X8

"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.03) with 1OYY (Global RMSD=2.23)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
K7XJL6	Genome polyprotein		OrthoMCL BLAST	MK-1220MK-7009CHEMBL2063087CHEMBL2063088...
K7XGM7	Genome polyprotein	Hepatitis C virus	OrthoMCL BLAST	US8741926, 47US8741926, 91US8741926, 55US8741926, 81...
A3EZJ3	Genome polyprotein	Hepatitis C virus	OrthoMCL BLAST	MLS000035179cid_654089MLS000039507MLS000081062...
A3EZI9	Genome polyprotein/Non-structural protein 4A	Hepatitis C virus	OrthoMCL BLAST	CHEMBL75558CHEMBL175386CHEMBL75558CHEMBL82642...
Q04045	Genome polyprotein	Hepatitis C virus	OrthoMCL BLAST	NS3 Protease Inhibitor, 1NS3 Protease Inhibitor, 2NS3 Protease Inhibitor, 3NS3 Protease Inhibitor, 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_103442

Most Similar Human Ortholog: Q9UJV9

TM-align score: 0.84 | RMSD: 2.31

Seq Identity: 0.57 | Length: 547 / 665

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000183258	DEAD-box helicase 41

Protein Information

Protein Length: 665 | Molecular Weight (kDa): 76.205

UniProt ID(s): A0A5K1K8X8, O97222, Q9U936

PDB ID(s): None

Isoelectric Point: 8.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	36	24	45
disruptive	17	52	41	112
missense	11	39	36	80

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	89	31	31
disruptive	30	140	75	162
missense	20	94	63	100

PlasmoDB Total SNPs: 112

Non-coding: 64 | Synonymous: 22 | Nonsynonymous: 26 | Stop Codon: 0