Genome location: Pf3D7_05_v3:1,170,170..1,174,394(+)
Genome classification: Core
Product Description: palmitoyltransferase DHHC7
SignalP Peptide: N/A
# Transmembrane Domains: 4
EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0020008 | rhoptry |
| Function | GO:0016746 | acyltransferase activity |
| Function | GO:0016409 | palmitoyltransferase activity |
| Function | GO:0019706 | protein-cysteine S-palmitoyltransferase activity |
| Process | GO:0018230 | peptidyl-L-cysteine S-palmitoylation |
| Process | GO:0018345 | protein palmitoylation |
| Process | GO:0006612 | protein targeting to membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.05 | 0.97 |
| Schizont | expressed | 0.76 | 0.57 |
| Gametocyte | expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0528400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.394 | MFS: -2.585 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1243000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1243000)
Modification: Tagged | RMgm-901
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3I3
"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.11) with 6BMM (Global RMSD=5.00)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q5W0Z9 | Palmitoyltransferase ZDHHC20 | OrthoMCL BLAST | CHEMBL118417CHEMBL5201039CHEMBL5186346CHEMBL5205503... |
Ortholog Group (OrthoMCL): OG6_100499
Most Similar Human Ortholog: Q96MV8
TM-align score: 0.78 | RMSD: 2.93
Seq Identity: 0.25 | Length: 291 / 406
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102383 | zinc finger DHHC-type palmitoyltransferase 15 |
| ENSG00000104219 | zinc finger DHHC-type palmitoyltransferase 2 |
| ENSG00000180776 | zinc finger DHHC-type palmitoyltransferase 20 |
Protein Length: 406 | Molecular Weight (kDa): 48.242
UniProt ID(s): Q8I3I3
PDB ID(s): None
Isoelectric Point: 9.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001594 | Palmitoyltransferase, DHHC domain |
| PFam | PF01529 | Palmitoyltransferase, DHHC domain |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 31 | 13 | 25 |
| disruptive | 12 | 28 | 13 | 47 |
| missense | 12 | 26 | 13 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 50 | 22 | 30 |
| disruptive | 18 | 67 | 37 | 101 |
| missense | 15 | 55 | 32 | 67 |
PlasmoDB Total SNPs: 398
Non-coding: 361 | Synonymous: 29 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23638681 | Global analysis of apicomplexan protein S-acyl transferases reveals an enzymeessential for invasion. | Frenal K, Tay CL, ..., Soldati-Favre D | 10.1111/tra.12081 |
| 27060339 | Study of Plasmodium falciparum DHHC palmitoyl transferases identifies a role forPfDHHC9 in gametocytogenesis. | Tay CL, Jones ML, ..., Rayner JC | 10.1111/cmi.12599 |
| 30761251 | Plasmodium palmitoylation machinery engineered in E. coli for high-throughputscreening of palmitoyl acyl-transferase inhibitors. | Yadav P, Ayana R, ..., Singh S | 10.1002/2211-5463.12564 |
| 27875558 | Palmitoylated APP Forms Dimers, Cleaved by BACE1 | Bhattacharyya R, Fenn RH, ..., Kovacs DM | 10.1371/journal.pone.0166400 |