PF3D7_0528800

Genome location: Pf3D7_05_v3:1,180,162..1,182,686(-)

Genome classification: Core

Function and Localization

Product Description: nuclear GTP-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.5.2 (Small monomeric GTPase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Function	GO:0003924	GTPase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.85	0.61
Trophozoite	expressed	0.55	0.70
Schizont	possibly expressed	0.13	0.92
Gametocyte	possibly expressed	0.49	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0528800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.17 | MFS: -3.22 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1243400)

Relative Growth Rate: 0.26 ± 0.58
Confidence: 2.48

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3H9

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=5.19) with 5DN8 (Global RMSD=12.82)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.5.2	small monomeric GTPase	GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533194

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	31	22	46
disruptive	10	59	52	93
missense	10	57	52	87

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	60	33	43
disruptive	22	165	82	154
missense	17	140	68	110

PlasmoDB Total SNPs: 95

Non-coding: 63 | Synonymous: 20 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 537 | Molecular Weight (kDa): 63.934

UniProt ID(s): Q8I3H9

PDB ID(s): None

Isoelectric Point: 10.04

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR014813	Guanine nucleotide-binding protein-like 3, N-terminal domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF08701	Guanine nucleotide-binding protein-like 3, N-terminal domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104