PF3D7_0529300 (TIC22)

Genome location: Pf3D7_05_v3:1,195,884..1,197,645(+)

Genome classification: Core

Function and Localization

Product Description: apicoplast TIC22 protein

SignalP Peptide: MCLLLFICLYFARGIYC

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0016021	integral component of membrane
Component	GO:0016020	membrane
Process	GO:0006457	protein folding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.04	0.98
Trophozoite	possibly expressed	0.34	0.79
Schizont	expressed	0.07	0.95
Gametocyte	expressed	0.17	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0529300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.449 | MFS: -2.708 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1243800)

Relative Growth Rate: 0.91 ± 0.13
Confidence: 5.44

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1243800)

Modification: Disrupted | RMgm-3385

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3H4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_111666

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	15	12	18
disruptive	4	27	21	38
missense	3	25	21	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	36	9	29
disruptive	9	70	38	85
missense	4	61	35	61

PlasmoDB Total SNPs: 111

Non-coding: 99 | Synonymous: 10 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 279 | Molecular Weight (kDa): 33.474

UniProt ID(s): Q8I3H4

PDB ID(s): 4E6Z

Isoelectric Point: 9.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007378	Tic22-like
PFam	PF04278	Tic22-like
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
19502580	Characterization of two putative protein translocation components in theapicoplast of Plasmodium falciparum.	Kalanon M, Tonkin CJ, McFadden GI	10.1128/EC.00061-09
22442242	Overproduction, purification and crystallization of PfTic22, a component of theimport apparatus from the apicoplast of Plasmodium falciparum.	Glaser S, Higgins MK	10.1107/S1744309112004952
23027875	Tic22 is an essential chaperone required for protein import into the apicoplast.	Glaser S, van Dooren GG, ..., Higgins MK	10.1074/jbc.M112.405100
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895