Genome location: Pf3D7_05_v3:1,311,992..1,315,355(+)
Genome classification: Core
Product Description: lysine-rich membrane-associated PHISTb protein
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0044164 | host cell cytosol |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0044538 | host cell periphery |
| Component | GO:0044228 | host cell surface |
| Component | GO:0020030 | infected host cell surface knob |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.44 | 0.83 |
| Trophozoite | expressed | 0.74 | 0.62 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | not expressed | 0.08 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0532400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.218 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3F0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 13 | 7 | 17 |
| disruptive | 37 | 68 | 13 | 59 |
| missense | 22 | 38 | 11 | 44 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 23 | 12 | 20 |
| disruptive | 52 | 107 | 43 | 137 |
| missense | 27 | 63 | 30 | 83 |
PlasmoDB Total SNPs: 176
Non-coding: 98 | Synonymous: 40 | Nonsynonymous: 38 | Stop Codon: 0
Protein Length: 528 | Molecular Weight (kDa): 61.086
UniProt ID(s): Q8I3F0
PDB ID(s): None
Isoelectric Point: 9.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR019111 | Plasmodium RESA, N-terminal |
| PFam | PF09687 | Plasmodium RESA, N-terminal |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 24706359 | A lysine-rich membrane-associated PHISTb protein involved in alteration of thecytoadhesive properties of Plasmodium falciparum-infected red blood cells. | Proellocks NI, Herrmann S, ..., Cooke BM | 10.1096/fj.14-250399 |
| 24983468 | A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 andcomigrates to knobs on the host cell surface. | Oberli A, Slater LM, ..., Vakonakis I | 10.1096/fj.14-256057 |
| 25106850 | A conserved domain targets exported PHISTb family proteins to the periphery ofPlasmodium infected erythrocytes. | Tarr SJ, Moon RW, Hardege I, Osborne AR | 10.1016/j.molbiopara.2014.07.011 |
| 26916885 | Plasmodium falciparum Plasmodium helical interspersed subtelomeric proteinscontribute to cytoadherence and anchor P. falciparum erythrocyte membrane protein1 to the host cell cytoskeleton. | Oberli A, Zurbrugg L, ..., Beck HP | 10.1111/cmi.12583 |
| 27022937 | The Plasmodium PHIST and RESA-Like Protein Families of Human and Rodent MalariaParasites. | Moreira CK, Naissant B, ..., Templeton TJ | 10.1371/journal.pone.0152510 |
| 28981210 | Proteome and Structural Organization of the Knob Complex on the Surface of thePlasmodium Infected Red Blood Cell. | Alampalli SV, Grover M, ..., Acharya P | 10.1002/prca.201600177 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33774908 | Characterisation of complexes formed by parasite proteins exported into the hostcell compartment of Plasmodium falciparum infected red blood cells. | Jonsdottir TK, Counihan NA, ..., Gilson PR | 10.1111/cmi.13332 |