Product Description: ATP-dependent RNA helicase MTR4
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031499 | TRAMP complex |
| Component | GO:0034399 | nuclear periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006401 | RNA catabolic process |
| Process | GO:0000460 | maturation of 5.8S rRNA |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.63 | 0.71 |
| Trophozoite | expressed | 0.48 | 0.74 |
| Schizont | not expressed | 0.10 | 0.95 |
| Gametocyte | expressed | 0.58 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0602100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.01 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0811100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSM1
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.75) with 6IEH (Global RMSD=2.46)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100340
Most Similar Human Ortholog: P42285
TM-align score: 0.74 | RMSD: 3.98
Seq Identity: 0.43 | Length: 836 / 1350
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000039123 | Mtr4 exosome RNA helicase |
| ENSG00000204351 | Ski2 like RNA helicase |
| ENSG00000206353 | Ski2 like RNA helicase |
| ENSG00000223493 | Ski2 like RNA helicase |
| ENSG00000225737 | Ski2 like RNA helicase |
| ENSG00000228896 | Ski2 like RNA helicase |
| ENSG00000232616 | Ski2 like RNA helicase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 26 | 62 | 43 | 86 |
| disruptive | 23 | 123 | 54 | 174 |
| missense | 20 | 116 | 49 | 142 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 37 | 155 | 54 | 75 |
| disruptive | 54 | 253 | 109 | 294 |
| missense | 43 | 200 | 93 | 192 |
PlasmoDB Total SNPs: 224
Non-coding: 123 | Synonymous: 58 | Nonsynonymous: 42 | Stop Codon: 1
Protein Length: 1350 | Molecular Weight (kDa): 157.309
UniProt ID(s): C6KSM1
PDB ID(s): None
Isoelectric Point: 6.47
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR012961 | ATP-dependent RNA helicase Ski2, C-terminal |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR016438 | ATP-dependent RNA helicase Ski2-like |
| InterPro | IPR025696 | rRNA-processing arch domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF08148 | ATP-dependent RNA helicase Ski2, C-terminal |
| PFam | PF13234 | rRNA-processing arch domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 32079470 | The cryptic unstable transcripts are associated with developmentally regulatedgene expression in blood-stage Plasmodium falciparum. | Yin S, Fan Y, ..., Zhang Q | 10.1080/15476286.2020.1732032 |
| 37667796 | The RNA helicases Dbp2 and Mtr4 regulate the expression of Xrn1-sensitive longnon-coding RNAs in yeast | Wery M, Szachnowski U, ..., Morillon A | 10.3389/frnar.2023.1244554 |
| 36861343 | In vivo characterization of the critical interaction between the RNA exosome andthe essential RNA helicase Mtr4 in Saccharomyces cerevisiae | Sterrett MC, Farchi D, ..., Corbett AH | 10.1093/g3journal/jkad049 |