Product Description: elongation factor G
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0003746 | translation elongation factor activity |
| Process | GO:0032543 | mitochondrial translation |
| Process | GO:0032790 | ribosome disassembly |
| Process | GO:0006414 | translational elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.21 | 0.90 |
| Trophozoite | expressed | 1.17 | 0.43 |
| Schizont | expressed | 0.17 | 0.90 |
| Gametocyte | expressed | 0.19 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0602400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.283 | MFS: -2.705 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0101200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSM4
"Best" AlphaFill ligand hit: GCP (phosphomethylphosphonic acid guanylate ester, Local RMSD=0.38) with 2J7K (Global RMSD=2.26)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103657
Most Similar Human Ortholog: D6RF75
TM-align score: 0.82 | RMSD: 1.49
Seq Identity: 0.53 | Length: 64 / 937
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000164347 | GTP dependent ribosome recycling factor mitochondrial 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 42 | 27 | 48 |
| disruptive | 10 | 51 | 29 | 65 |
| missense | 10 | 49 | 29 | 58 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 90 | 31 | 48 |
| disruptive | 21 | 96 | 50 | 127 |
| missense | 19 | 84 | 44 | 103 |
PlasmoDB Total SNPs: 148
Non-coding: 101 | Synonymous: 31 | Nonsynonymous: 15 | Stop Codon: 1
Protein Length: 937 | Molecular Weight (kDa): 108.308
UniProt ID(s): C5J0A0, C5J0A3, C5J0B1, C5J0B2, C5J0B6, C5J0B9, C5J0C0, C5J0D1, C5J0D7, C5J0D9, C6KSM4
PDB ID(s): None
Isoelectric Point: 8.71
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000640 | Elongation factor EFG, domain V-like |
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR004161 | Translation elongation factor EFTu-like, domain 2 |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR005517 | Translation elongation factor EFG/EF2, domain IV |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031157 | Tr-type G domain, conserved site |
| InterPro | IPR035647 | EF-G domain III/V-like |
| InterPro | IPR035649 | EFG, domain V |
| InterPro | IPR041095 | Elongation Factor G, domain II |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| PFam | PF00679 | Elongation factor EFG, domain V-like |
| PFam | PF03144 | Translation elongation factor EFTu-like, domain 2 |
| PFam | PF03764 | Translation elongation factor EFG/EF2, domain IV |
| PFam | PF14492 | Elongation Factor G, domain II |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| Superfamily | SSF54980 | EF-G domain III/V-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 21695207 | Characterization of two malaria parasite organelle translation elongation factorG proteins: the likely targets of the anti-malarial fusidic acid. | Johnson RA, McFadden GI, Goodman CD | 10.1371/journal.pone.0020633 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 36984696 | 3-Amino-Substituted Analogues of Fusidic Acid as Membrane-Active AntibacterialCompounds | Salimova EV, Mozgovoj OS, ..., Parfenova LV | 10.3390/membranes13030309 |
| 37648010 | Active role of the protein translation machinery in protecting against stresstolerance in Synechococcus elongatus PCC7942 | Ngoennet S, Sirisattha S, ..., Waditee-Sirisattha R | 10.1016/j.abb.2023.109734 |