PF3D7_0602800 (JmjC2)

Genome location: Pf3D7_06_v3:115,789..118,379(+)

Genome classification: Core

Function and Localization

Product Description: JmjC domain-containing protein 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.14.11.27 ([Histone H3]-dimethyl-L-lysine(36) demethylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016706	2-oxoglutarate-dependent dioxygenase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.31	0.85
Trophozoite	possibly expressed	0.16	0.91
Schizont	possibly expressed	0.05	0.97
Gametocyte	not expressed	0.21	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0602800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -1.838 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0101600)

Relative Growth Rate: 0.86 ± 0.18
Confidence: 4.86

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0101600)

Modification: Disrupted | RMgm-1608

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSM8

"Best" AlphaFill ligand hit: HQY (8-hydroxyquinoline, Local RMSD=0.13) with 3KCY (Global RMSD=7.24)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.14.11.27	[histone H3]-dimethyl-L-lysine36 demethylase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106314

Most Similar Human Ortholog: A2RUC4

TM-align score: 0.90 | RMSD: 2.64

Seq Identity: 0.26 | Length: 314 / 646

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000162971	tRNA-yW synthesizing protein 5

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	28	13	36
disruptive	27	86	48	109
missense	24	79	42	89

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	53	24	42
disruptive	50	207	85	185
missense	39	161	71	118

PlasmoDB Total SNPs: 115

Non-coding: 30 | Synonymous: 49 | Nonsynonymous: 35 | Stop Codon: 1

Protein Information

Protein Length: 646 | Molecular Weight (kDa): 77.767

UniProt ID(s): C6KSM8

PDB ID(s): None

Isoelectric Point: 10.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR041667	Cupin-like domain 8
PFam	PF13621	Cupin-like domain 8
Superfamily	SSF51197	N/A