Product Description: phenylalanine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.20 (Phenylalanine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004826 | phenylalanine-tRNA ligase activity |
| Process | GO:0006432 | phenylalanyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.24 | 0.85 |
| Schizont | expressed | 0.05 | 0.96 |
| Gametocyte | expressed | 0.15 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0603700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.137 | MFS: -2.866 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSN5
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.79) with 3PCO (Global RMSD=1.61)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.20 | phenylalanine-tRNA ligase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O95363 | Phenylalanine--tRNA ligase, mitochondrial | Homo sapiens | OMA, OrthoDB | CHEMBL25737CHEMBL27258CHEMBL284654CHEMBL281860... |
Ortholog Group (OrthoMCL): OG6_102538
Most Similar Human Ortholog: O95363
TM-align score: 0.73 | RMSD: 1.69
Seq Identity: 0.40 | Length: 339 / 443
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000145982 | phenylalanyl-tRNA synthetase 2, mitochondrial |
Protein Length: 443 | Molecular Weight (kDa): 53.336
UniProt ID(s): C6KSN5
PDB ID(s): None
Isoelectric Point: 8.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002319 | Phenylalanyl-tRNA synthetase |
| InterPro | IPR004530 | Phenylalanyl-tRNA synthetase, class IIc, mitochondrial |
| InterPro | IPR005121 | Ferrodoxin-fold anticodon-binding domain |
| InterPro | IPR036690 | Ferrodoxin-fold anticodon-binding domain superfamily |
| PFam | PF01409 | Phenylalanyl-tRNA synthetase |
| PFam | PF03147 | Ferrodoxin-fold anticodon-binding domain |
| Superfamily | SSF54991 | Ferrodoxin-fold anticodon-binding domain superfamily |
| Superfamily | SSF55681 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 17 | 15 | 29 |
| disruptive | 4 | 25 | 14 | 42 |
| missense | 4 | 25 | 14 | 36 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 48 | 17 | 28 |
| disruptive | 6 | 61 | 31 | 85 |
| missense | 6 | 55 | 29 | 56 |
PlasmoDB Total SNPs: 74
Non-coding: 53 | Synonymous: 16 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25391660 | Plasmodium falciparum mitochondria import tRNAs along with an activephenylalanyl-tRNA synthetase. | Sharma A, Sharma A | 10.1042/BJ20140998 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |