PF3D7_0604500

Genome location: Pf3D7_06_v3:188,237..198,546(+)

Genome classification: Core

Function and Localization

Product Description: GYF domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0003729	mRNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	2.74	0.13
Trophozoite	expressed	2.16	0.15
Schizont	expressed	1.57	0.31
Gametocyte	expressed	1.73	0.25

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0604500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.601 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0103300)

Modification: Disrupted | RMgm-1615

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532435

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	30	163	87	174
disruptive	150	529	264	616
missense	74	367	192	447

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	58	332	138	197
disruptive	320	1113	457	1046
missense	144	754	330	611

PlasmoDB Total SNPs: 605

Non-coding: 182 | Synonymous: 295 | Nonsynonymous: 128 | Stop Codon: 0

Protein Information

Protein Length: 2874 | Molecular Weight (kDa): 334.41

UniProt ID(s): C6KSP3, Q25768, Q25772

PDB ID(s): None

Isoelectric Point: 8.73

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003169	GYF domain
InterPro	IPR035445	GYF-like domain superfamily
PFam	PF02213	GYF domain
Superfamily	SSF55277	GYF-like domain superfamily

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
27381095	The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.	Bunnik EM, Batugedara G, ..., Le Roch KG	10.1186/s13059-016-1014-0
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596