PF3D7_0604600

Genome location: Pf3D7_06_v3:198,862..204,355(+)

Genome classification: Core

Function and Localization

Product Description: DNA helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0043596	nuclear replication fork
Component	GO:0005634	nucleus
Function	GO:0036310	ATP-dependent DNA/DNA annealing activity
Function	GO:0008094	ATP-dependent activity, acting on DNA
Function	GO:0003678	DNA helicase activity
Function	GO:0004520	endodeoxyribonuclease activity
Process	GO:0006281	DNA repair
Process	GO:0036292	DNA rewinding
Process	GO:0006974	cellular response to DNA damage stimulus
Process	GO:0031297	replication fork processing
Process	GO:0048478	replication fork protection

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.19	0.90
Trophozoite	possibly expressed	0.35	0.80
Schizont	not expressed	0.12	0.93
Gametocyte	not expressed	0.35	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0604600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.592 | #Insertions: 5

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSP4

"Best" AlphaFill ligand hit: KH2 (3-(1-methylpiperidinium-1-yl)propane-1-sulfonate, Local RMSD=1.92) with 5HZR (Global RMSD=5.15)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_187368

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	72	48	108
disruptive	34	165	142	277
missense	29	153	135	247

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	159	69	121
disruptive	88	423	230	455
missense	55	362	190	317

PlasmoDB Total SNPs: 179

Non-coding: 78 | Synonymous: 64 | Nonsynonymous: 35 | Stop Codon: 2

Protein Information

Protein Length: 1712 | Molecular Weight (kDa): 206.428

UniProt ID(s): C6KSP4

PDB ID(s): None

Isoelectric Point: 10.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000330	SNF2-related, N-terminal domain
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR002711	HNH endonuclease
InterPro	IPR003615	HNH nuclease
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00176	SNF2-related, N-terminal domain
PFam	PF00271	Helicase, C-terminal
PFam	PF01844	HNH endonuclease
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase