Product Description: glyoxalase I-like protein GILP
SignalP Peptide: MKLFVVVIFVLFILLKRYKCLS
# Transmembrane Domains: 0
EC Numbers: 4.4.1.5 (Lactoylglutathione lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004462 | lactoylglutathione lyase activity |
| Process | GO:0019243 | methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione |
| Process | GO:0009438 | methylglyoxal metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.50 | 0.70 |
| Schizont | expressed | 0.14 | 0.91 |
| Gametocyte | expressed | 0.33 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0604700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.821 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSP5
"Best" AlphaFill ligand hit: GSH (glutathione, Local RMSD=4.02) with 6BNN (Global RMSD=4.99)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.4.1.5 | lactoylglutathione lyase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P50107 | Glyoxalase I | Saccharomyces cerevisiae | OrthoDB | CHEMBL128872CHEMBL221011CHEMBL218644CHEMBL128872... |
| Q04760 | Lactoylglutathione lyase | OrthoDB | CHEMBL218644CHEMBL159603CHEMBL218644S-(N-Methyl-N-hydroxycarbomyl)ethylglutathione... | |
| Q9CPU0 | Lactoylglutathione lyase | OrthoDB | CHEMBL1234300glycyrrhetinic acidCHEMBL4584432 |
Ortholog Group (OrthoMCL): OG6_105403
Most Similar Human Ortholog: I3L3Q4
TM-align score: 0.76 | RMSD: 2.44
Seq Identity: 0.14 | Length: 197 / 307
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000167699 | glyoxalase domain containing 4 |
Protein Length: 307 | Molecular Weight (kDa): 35.838
UniProt ID(s): C6KSP5
PDB ID(s): None
Isoelectric Point: 8.51
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004360 | Glyoxalase/fosfomycin resistance/dioxygenase domain |
| InterPro | IPR029068 | Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase |
| PFam | PF00903 | Glyoxalase/fosfomycin resistance/dioxygenase domain |
| Superfamily | SSF54593 | Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 23 | 17 | 18 |
| disruptive | 3 | 28 | 12 | 48 |
| missense | 3 | 28 | 12 | 46 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 45 | 14 | 21 |
| disruptive | 12 | 49 | 27 | 72 |
| missense | 9 | 44 | 27 | 64 |
PlasmoDB Total SNPs: 131
Non-coding: 110 | Synonymous: 15 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 23678649 | Effects of low protein diet and pregnancy on course of Plasmodium bergheiinfection in mice | Rabiu OR, Arinola OG, Odaibo AB, Ademowo OG | https://pubmed.ncbi.nlm.nih.gov/23678649/ |