PF3D7_0605600

Genome location: Pf3D7_06_v3:228,132..234,835(-)

Genome classification: Core

Function and Localization

Product Description: nucleoside diphosphate kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.4.6 (Nucleoside-diphosphate kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004550	nucleoside diphosphate kinase activity
Process	GO:0006241	CTP biosynthetic process
Process	GO:0006183	GTP biosynthetic process
Process	GO:0006228	UTP biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.07	0.96
Trophozoite	not expressed	0.04	0.98
Schizont	not expressed	0.15	0.91
Gametocyte	expressed	0.85	0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0605600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.684 | #Insertions: 8

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSQ4

"Best" AlphaFill ligand hit: TAM (tris(hydroxyethyl)aminomethane, Local RMSD=0.01) with 6QA2 (Global RMSD=2.04)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.4.6	nucleoside-diphosphate kinase	ATPUDPAMPGDPGMPEDTAcAMPcGMPdTDPDTNBdCDPMgADP-...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_107372

Most Similar Human Ortholog: F8WEA2

TM-align score: 0.50 | RMSD: 3.24

Seq Identity: 0.03 | Length: 39 / 1828

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000086288	NME/NM23 family member 8
ENSG00000181322	NME/NM23 family member 9

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	25	71	51	115
disruptive	111	312	135	310
missense	70	263	123	288

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	37	137	82	114
disruptive	173	526	199	402
missense	102	467	180	352

PlasmoDB Total SNPs: 273

Non-coding: 79 | Synonymous: 88 | Nonsynonymous: 106 | Stop Codon: 0

Protein Information

Protein Length: 1828 | Molecular Weight (kDa): 219.869

UniProt ID(s): C6KSQ4

PDB ID(s): None

Isoelectric Point: 7.57

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001564	Nucleoside diphosphate kinase
InterPro	IPR034907	Nucleoside diphosphate kinase-like domain
InterPro	IPR036850	Nucleoside diphosphate kinase-like domain superfamily
PFam	PF00334	Nucleoside diphosphate kinase-like domain
Superfamily	SSF54919	Nucleoside diphosphate kinase-like domain superfamily