Product Description: DNA repair protein RAD50, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030870 | Mre11 complex |
| Component | GO:0000794 | condensed nuclear chromosome |
| Component | GO:0005634 | nucleus |
| Component | GO:0035861 | site of double-strand break |
| Function | GO:0051880 | G-quadruplex DNA binding |
| Function | GO:0004017 | adenylate kinase activity |
| Function | GO:0003690 | double-stranded DNA binding |
| Function | GO:0003691 | double-stranded telomeric DNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0000014 | single-stranded DNA endodeoxyribonuclease activity |
| Function | GO:0043047 | single-stranded telomeric DNA binding |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0070192 | chromosome organization involved in meiotic cell cycle |
| Process | GO:0006302 | double-strand break repair |
| Process | GO:0090305 | nucleic acid phosphodiester bond hydrolysis |
| Process | GO:0016233 | telomere capping |
| Process | GO:0000722 | telomere maintenance via recombination |
| Process | GO:0007004 | telomere maintenance via telomerase |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 1.28 | 0.49 |
| Trophozoite | expressed | 1.77 | 0.26 |
| Schizont | expressed | 0.67 | 0.67 |
| Gametocyte | expressed | 0.80 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0605800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.031 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0104600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0104600)
Modification: Disrupted | RMgm-1618
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSQ6
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.08) with 4NCJ (Global RMSD=3.24)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101667
Most Similar Human Ortholog: E9PM98
TM-align score: 0.94 | RMSD: 0.77
Seq Identity: 0.44 | Length: 100 / 2236
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000113522 | RAD50 double strand break repair protein |
| ENSG00000283782 | novel protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 81 | 59 | 125 |
| disruptive | 87 | 231 | 116 | 315 |
| missense | 53 | 174 | 105 | 269 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 35 | 163 | 85 | 128 |
| disruptive | 163 | 446 | 208 | 413 |
| missense | 78 | 338 | 174 | 333 |
PlasmoDB Total SNPs: 505
Non-coding: 168 | Synonymous: 179 | Nonsynonymous: 156 | Stop Codon: 2
Protein Length: 2236 | Molecular Weight (kDa): 267.956
UniProt ID(s): C6KSQ6
PDB ID(s): None
Isoelectric Point: 8.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR038729 | Rad50/SbcC-type AAA domain |
| PFam | PF13476 | Rad50/SbcC-type AAA domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF58104 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25938776 | Identification of Plasmodium falciparum DNA Repair Protein Mre11 with anEvolutionarily Conserved Nuclease Function. | Badugu SB, Nabi SA, ..., Bhattacharyya MK | 10.1371/journal.pone.0125358 |