Product Description: ubiquitin-conjugating enzyme E2 PEX4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme);6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Process | GO:0000209 | protein polyubiquitination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.21 | 0.86 |
| Gametocyte | expressed | 0.16 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0606200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.153 | MFS: -2.908 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSR0
"Best" AlphaFill ligand hit: BU3 ((r,r)-2,3-butanediol, Local RMSD=0.02) with 1Z2U (Global RMSD=0.87)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.23 | E2 ubiquitin-conjugating enzyme | CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione... |
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P63146 | Ubiquitin-conjugating enzyme E2 B | OrthoDB | CHEMBL3787660 | |
| P51965 | Ubiquitin-conjugating enzyme E2 E1 | OrthoDB | US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004... | |
| P61088 | Ubiquitin-conjugating enzyme E2 N | Homo sapiens | OrthoDB | MLS000074439MLS000032400MLS000041097MLS000055488... |
Protein Length: 155 | Molecular Weight (kDa): 17.815
UniProt ID(s): C6KSR0
PDB ID(s): None
Isoelectric Point: 5.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000608 | Ubiquitin-conjugating enzyme E2 |
| InterPro | IPR016135 | Ubiquitin-conjugating enzyme/RWD-like |
| PFam | PF00179 | Ubiquitin-conjugating enzyme E2 |
| Superfamily | SSF54495 | Ubiquitin-conjugating enzyme/RWD-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 3 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 2 | 1 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 156
Non-coding: 152 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28552664 | Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbora subset of peroxisomal membrane proteins | Wroblewska JP, Cruz-Zaragoza LD, ..., van der Klei IJ | 10.1016/j.bbamcr.2017.05.021 |