PF3D7_0606500 (PTB)

Genome location: Pf3D7_06_v3:264,901..269,898(-)

Genome classification: Core

Function and Localization

Product Description: polypyrimidine tract-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0003729	mRNA binding
Function	GO:0008187	poly-pyrimidine tract binding
Process	GO:0008380	RNA splicing
Process	GO:0043484	regulation of RNA splicing
Process	GO:0006417	regulation of translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.51	0.75
Trophozoite	expressed	0.43	0.74
Schizont	expressed	0.13	0.92
Gametocyte	expressed	0.28	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0606500

Resistome Missense Mutations: V492D

Resistome Compounds with Missense Mutations: NCP26

Resistome # Samples with Disruptive Mutations: 6 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.487 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0105200)

Relative Growth Rate: 0.86 ± 0.16
Confidence: 5.10

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0105200)

Modification: Disrupted | RMgm-1623

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSR3

"Best" AlphaFill ligand hit: GTA (p1-7-methylguanosine-p3-adenosine-5',5'-triphosphate, Local RMSD=0.23) with 6D0Y (Global RMSD=4.95)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101075

Most Similar Human Ortholog: A0A0D9SF20

TM-align score: 0.90 | RMSD: 2.12

Seq Identity: 0.26 | Length: 194 / 663

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000011304	polypyrimidine tract binding protein 1
ENSG00000117569	polypyrimidine tract binding protein 2
ENSG00000119314	polypyrimidine tract binding protein 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	38	21	47
disruptive	18	71	37	76
missense	11	60	33	66

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	82	22	47
disruptive	28	164	68	172
missense	17	130	52	125

PlasmoDB Total SNPs: 307

Non-coding: 259 | Synonymous: 32 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 663 | Molecular Weight (kDa): 77.219

UniProt ID(s): C6KSR3

PDB ID(s): None

Isoelectric Point: 8.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR021790	PTBP1, RNA recognition motif 2-like
InterPro	IPR035979	RNA-binding domain superfamily
PFam	PF00076	RNA recognition motif domain
PFam	PF11835	PTBP1, RNA recognition motif 2-like
PFam	PF13893
Superfamily	SSF54928	RNA-binding domain superfamily