About Gene List

PF3D7_0607600 (SAS6)

Genome location: Pf3D7_06_v3:311,667..318,394(-)

Genome classification: Core

Function and Localization

Product Description: spindle assembly abnormal protein 6, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005814 centriole
Component GO:0005634 nucleus
Process GO:0007052 mitotic spindle organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.03 0.98
Trophozoite possibly expressed 0.34 0.79
Schizont expressed 0.61 0.66
Gametocyte possibly expressed 0.26 0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0607600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.782 | #Insertions: 6

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0106200)

  • Relative Growth Rate: 1.02 ± 0.10
  • Confidence: 5.91

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0106200)

Modification: Disrupted | RMgm-5154

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSS4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104016

Most Similar Human Ortholog: Q6UVJ0

TM-align score: 0.45 | RMSD: 6.66

Seq Identity: 0.14 | Length: 418 / 861

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000156876 SAS-6 centriolar assembly protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 2 41 26 55
disruptive 22 68 35 118
missense 15 60 29 98

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 81 47 64
disruptive 36 170 100 218
missense 24 135 81 139

PlasmoDB Total SNPs: 265

Non-coding: 206 | Synonymous: 31 | Nonsynonymous: 28 | Stop Codon: 0

Protein Information

Protein Length: 861 | Molecular Weight (kDa): 101.236

UniProt ID(s): C6KSS4

PDB ID(s): None

Isoelectric Point: 6.24

Protein Domain Annotations:

Source Family ID Description
InterPro IPR032396 Spindle assembly abnormal protein 6, N-terminal
PFam PF16531 Spindle assembly abnormal protein 6, N-terminal
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
25154861 An essential role of the basal body protein SAS-6 in Plasmodium male gametedevelopment and malaria transmission. Marques SR, Ramakrishnan C, ..., Sinden RE 10.1111/cmi.12355
35077503 Expansion microscopy of Plasmodium gametocytes reveals the molecular architectureof a bipartite microtubule organisation centre coordinating mitosis with axonemeassembly Rashpa R, Brochet M 10.1371/journal.ppat.1010223