PF3D7_0609200

Genome location: Pf3D7_06_v3:392,371..394,770(+)

Genome classification: Core

Function and Localization

Product Description: citrate synthase-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.3.3.1 (Citrate (Si)-synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005759	mitochondrial matrix
Function	GO:0004108	citrate (Si)-synthase activity
Process	GO:0005975	carbohydrate metabolic process
Process	GO:0006099	tricarboxylic acid cycle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.15	0.93
Trophozoite	expressed	0.19	0.88
Schizont	possibly expressed	0.11	0.92
Gametocyte	possibly expressed	0.27	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0609200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.049 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0107800)

Relative Growth Rate: 0.18 ± 0.09
Confidence: 6.29

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KST9

"Best" AlphaFill ligand hit: OAA (oxaloacetate ion, Local RMSD=0.06) with 2R26 (Global RMSD=3.56)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.3.1	citrate (Si)-synthase	ATPSDSNADHHgCl2CuSO4citrateMaleateOuabainD-serineDL-malatepropionateMaleic acid...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_215332

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	20	16	17
disruptive	5	43	32	66
missense	5	42	30	62

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	36	21	18
disruptive	13	120	55	98
missense	11	100	49	74

PlasmoDB Total SNPs: 151

Non-coding: 131 | Synonymous: 10 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 465 | Molecular Weight (kDa): 56.204

UniProt ID(s): C6KST9

PDB ID(s): None

Isoelectric Point: 9.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002020	Citrate synthase
InterPro	IPR036969	Citrate synthase superfamily
PFam	PF00285	Citrate synthase
Superfamily	SSF48256	Citrate synthase superfamily