PF3D7_0609400 (CLS)

Genome location: Pf3D7_06_v3:399,357..402,112(-)

Genome classification: Core

Function and Localization

Product Description: mitochondrial cardiolipin synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.8.- (Transferases for other substituted phosphate groups.);2.7.8.41 (Cardiolipin synthase (CMP-forming))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.07	0.96
Trophozoite	no data	0.31	0.81
Schizont	no data	0.06	0.96
Gametocyte	no data	0.57	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0609400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.132 | MFS: -2.943 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSU1

"Best" AlphaFill ligand hit: STU (staurosporine, Local RMSD=3.92) with 4MVF (Global RMSD=20.53)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.8.-	Ex: UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase	ATPADPUDPAMPGTPCTPUTPCDPTTPCMPNEMUMP...
2.7.8.41	cardiolipin synthase (CMP-forming)	EDTATriton X-100CDP-dioleoylglycerol1,2-dioleoylphosphatidylglycerol

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106810

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	20	12	35
disruptive	5	31	24	60
missense	5	29	23	54

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	42	24	35
disruptive	16	89	48	100
missense	8	73	41	83

PlasmoDB Total SNPs: 94

Non-coding: 66 | Synonymous: 20 | Nonsynonymous: 6 | Stop Codon: 2

Protein Information

Protein Length: 603 | Molecular Weight (kDa): 71.207

UniProt ID(s): C6KSU1

PDB ID(s): None

Isoelectric Point: 9.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001736	Phospholipase D/Transphosphatidylase
InterPro	IPR025202	Phospholipase D-like domain
PFam	PF13091	Phospholipase D-like domain
Superfamily	SSF56024	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21