Product Description: palmitoyltransferase DHHC2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 4
EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0070258 | inner membrane pellicle complex |
| Component | GO:0016021 | integral component of membrane |
| Function | GO:0003676 | nucleic acid binding |
| Function | GO:0016409 | palmitoyltransferase activity |
| Function | GO:0019706 | protein-cysteine S-palmitoyltransferase activity |
| Process | GO:0018230 | peptidyl-L-cysteine S-palmitoylation |
| Process | GO:0018345 | protein palmitoylation |
| Process | GO:0006612 | protein targeting to membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.01 | 1.00 |
| Trophozoite | no data | 0.01 | 0.99 |
| Schizont | no data | 0.16 | 0.88 |
| Gametocyte | no data | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0609800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.48 | MFS: -2.491 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1243000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0108300)
Modification: Tagged | RMgm-5181
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSU5
"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.22) with 6BMM (Global RMSD=5.96)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100499
Most Similar Human Ortholog: Q5W0Z9
TM-align score: 0.82 | RMSD: 2.57
Seq Identity: 0.30 | Length: 258 / 284
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102383 | zinc finger DHHC-type palmitoyltransferase 15 |
| ENSG00000104219 | zinc finger DHHC-type palmitoyltransferase 2 |
| ENSG00000180776 | zinc finger DHHC-type palmitoyltransferase 20 |
Protein Length: 284 | Molecular Weight (kDa): 33.147
UniProt ID(s): C6KSU5
PDB ID(s): None
Isoelectric Point: 8.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001594 | Palmitoyltransferase, DHHC domain |
| PFam | PF01529 | Palmitoyltransferase, DHHC domain |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 9 | 4 | 11 |
| disruptive | 0 | 4 | 2 | 10 |
| missense | 0 | 4 | 2 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 22 | 10 | 19 |
| disruptive | 2 | 17 | 23 | 75 |
| missense | 0 | 11 | 19 | 44 |
PlasmoDB Total SNPs: 202
Non-coding: 186 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 26526684 | The Plasmodium palmitoyl-S-acyl-transferase DHHC2 is essential for ookinetemorphogenesis and malaria transmission. | Santos JM, Kehrer J, ..., Mair GR | 10.1038/srep16034 |
| 32395856 | A protein palmitoylation cascade regulates microtubule cytoskeleton integrity inPlasmodium. | Wang X, Qian P, ..., Yuan J | 10.15252/embj.2019104168 |
| 22034844 | DHHC2 is a protein S-acyltransferase for Lck | Zeidman R, Buckland G, ..., Magee AI | 10.3109/09687688.2011.630682 |
| 35775739 | Inner membrane complex proteomics reveals a palmitoylation regulation criticalfor intraerythrocytic development of malaria parasite | Qian P, Wang X, ..., Yuan J | 10.7554/eLife.77447 |
| 30965003 | Palmitoylation by Multiple DHHC Enzymes Enhances Dopamine Transporter Functionand Stability | Bolland DE, Moritz AE, ..., Foster JD | 10.1021/acschemneuro.8b00558 |
| 38107792 | Dopamine transporter membrane mobility is bidirectionally regulated by phosphorylation and palmitoylation | Shetty M, Bolland DE, ..., Vaughan RA | 10.1016/j.crphys.2023.100106 |
| 25589740 | The palmitoyl acyltransferase DHHC2 regulates recycling endosome exocytosis andsynaptic potentiation through palmitoylation of AKAP79/150 | Woolfrey KM, Sanderson JL, Dell'Acqua ML | 10.1523/JNEUROSCI.2243-14.2015 |