About Gene List

PF3D7_0609800 (DHHC2)

Genome location: Pf3D7_06_v3:420,422..423,142(-)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005794 Golgi apparatus
Component GO:0005737 cytoplasm
Component GO:0005783 endoplasmic reticulum
Component GO:0070258 inner membrane pellicle complex
Component GO:0016021 integral component of membrane
Function GO:0003676 nucleic acid binding
Function GO:0016409 palmitoyltransferase activity
Function GO:0019706 protein-cysteine S-palmitoyltransferase activity
Process GO:0018230 peptidyl-L-cysteine S-palmitoylation
Process GO:0018345 protein palmitoylation
Process GO:0006612 protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite no data N/A N/A
Ring no data 0.01 1.00
Trophozoite no data 0.01 0.99
Schizont no data 0.16 0.88
Gametocyte no data 0.06 0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0609800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.48 | MFS: -2.491 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1243000)

  • Relative Growth Rate: 0.10 ± 0.08
  • Confidence: 6.35

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0108300)

Modification: Tagged | RMgm-5181

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSU5

"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.22) with 6BMM (Global RMSD=5.96)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.3.1.- Ex: [myelin-proteolipid] O-palmitoyltransferase CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225 protein S-acyltransferase stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
Q9UIJ5 Palmitoyltransferase ZDHHC2 OrthoDB CHEMBL118417CHEMBL5201039CHEMBL5199102CHEMBL5186633
Q5W0Z9 Palmitoyltransferase ZDHHC20 OrthoDB, OrthoMCL BLAST CHEMBL118417CHEMBL5201039CHEMBL5186346CHEMBL5205503...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100499

Most Similar Human Ortholog: Q5W0Z9

TM-align score: 0.82 | RMSD: 2.57

Seq Identity: 0.30 | Length: 258 / 284

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000102383 zinc finger DHHC-type palmitoyltransferase 15
ENSG00000104219 zinc finger DHHC-type palmitoyltransferase 2
ENSG00000180776 zinc finger DHHC-type palmitoyltransferase 20

Protein Information

Protein Length: 284 | Molecular Weight (kDa): 33.147

UniProt ID(s): C6KSU5

PDB ID(s): None

Isoelectric Point: 8.87

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001594 Palmitoyltransferase, DHHC domain
PFam PF01529 Palmitoyltransferase, DHHC domain
Superfamily N/A N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 2 9 4 11
disruptive 0 4 2 10
missense 0 4 2 9

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 2 22 10 19
disruptive 2 17 23 75
missense 0 11 19 44

PlasmoDB Total SNPs: 202

Non-coding: 186 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
26526684 The Plasmodium palmitoyl-S-acyl-transferase DHHC2 is essential for ookinetemorphogenesis and malaria transmission. Santos JM, Kehrer J, ..., Mair GR 10.1038/srep16034
32395856 A protein palmitoylation cascade regulates microtubule cytoskeleton integrity inPlasmodium. Wang X, Qian P, ..., Yuan J 10.15252/embj.2019104168
22034844 DHHC2 is a protein S-acyltransferase for Lck Zeidman R, Buckland G, ..., Magee AI 10.3109/09687688.2011.630682
35775739 Inner membrane complex proteomics reveals a palmitoylation regulation criticalfor intraerythrocytic development of malaria parasite Qian P, Wang X, ..., Yuan J 10.7554/eLife.77447
30965003 Palmitoylation by Multiple DHHC Enzymes Enhances Dopamine Transporter Functionand Stability Bolland DE, Moritz AE, ..., Foster JD 10.1021/acschemneuro.8b00558
38107792 Dopamine transporter membrane mobility is bidirectionally regulated by phosphorylation and palmitoylation Shetty M, Bolland DE, ..., Vaughan RA 10.1016/j.crphys.2023.100106
25589740 The palmitoyl acyltransferase DHHC2 regulates recycling endosome exocytosis andsynaptic potentiation through palmitoylation of AKAP79/150 Woolfrey KM, Sanderson JL, Dell'Acqua ML 10.1523/JNEUROSCI.2243-14.2015