PF3D7_0610500

Genome location: Pf3D7_06_v3:444,549..446,775(-)

Genome classification: Core

Function and Localization

Product Description: peptidyl-tRNA hydrolase PTRHD1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.95
Trophozoite	not expressed	0.17	0.90
Schizont	not expressed	0.01	0.99
Gametocyte	not expressed	0.03	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0610500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.138 | MFS: -3.033 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0108900)

Relative Growth Rate: 1.04 ± 0.16
Confidence: 5.10

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0108900)

Modification: Disrupted | RMgm-1649

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSV1

"Best" AlphaFill ligand hit: UD1 (uridine-diphosphate-n-acetylglucosamine, Local RMSD=0.70) with 5BJV (Global RMSD=13.89)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.29	aminoacyl-tRNA hydrolase	EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104361

Most Similar Human Ortholog: Q6GMV3

TM-align score: 0.90 | RMSD: 1.72

Seq Identity: 0.35 | Length: 119 / 123

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000184924	peptidyl-tRNA hydrolase domain containing 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 81

Non-coding: 71 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 123 | Molecular Weight (kDa): 14.457

UniProt ID(s): C6KSV1

PDB ID(s): None

Isoelectric Point: 7.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002833	Peptidyl-tRNA hydrolase, PTH2
InterPro	IPR023476	Peptidyl-tRNA hydrolase II domain superfamily
InterPro	IPR042237	Putative peptidyl-tRNA hydrolase PTRHD1
PFam	PF01981	Peptidyl-tRNA hydrolase, PTH2
Superfamily	SSF102462	Peptidyl-tRNA hydrolase II domain superfamily