Product Description: calcium-dependent protein kinase 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0009931 | calcium-dependent protein serine/threonine kinase activity |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.30 | 0.81 |
| Schizont | expressed | 0.22 | 0.84 |
| Gametocyte | expressed | 0.26 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0610600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.197 | #Insertions: 7
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1351500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P49138 | MAP kinase-activated protein kinase 2 | Mus musculus | OrthoMCL BLAST | CHEMBL1235213 |
| Q6S4W0 | Calcium-dependent protein kinase 1 | OrthoMCL BLAST | CHEMBL2069957CHEMBL2069954CHEMBL1784959CHEMBL1784960... | |
| A0A7S7LIM2 | Uncharacterized protein | OrthoMCL BLAST | CHEMBL3108912CHEMBL2069957CHEMBL3108913CHEMBL2070049... | |
| Q5CTY5 | Calcium/calmodulin dependent protein kinase with a kinas domain and 4 calmodulin-like EF hands | OrthoMCL BLAST | CHEMBL2069954CHEMBL1784959CHEMBL1784960CHEMBL1784961... | |
| A0A0F7UUA6 | CAM kinase, CDPK family TgCDPK1 | OrthoMCL BLAST | US10632122, Example 1US10632122, Example 2US10632122, Example 3US10632122, Example 4... | |
| Q9BJF5 | Calmodulin-domain protein kinase 1 | OrthoMCL BLAST | CHEMBL3108912CHEMBL2069957CHEMBL3108913CHEMBL2070049... | |
| P49137 | MAP kinase-activated protein kinase 2 | Homo sapiens | OrthoMCL BLAST | Pyrrolopyridinone, 1JMC502647 Compound 7Pyrrolopyridine, 9Pyrrolopyridine, 10... |
| A3FQ16 | Calmodulin-domain protein kinase 1, putative | OrthoMCL BLAST | CHEMBL2069954CHEMBL1784959CHEMBL1784960CHEMBL1784961... | |
| O88351 | Inhibitor of nuclear factor kappa-B kinase subunit beta | Mus musculus | OrthoMCL BLAST | CHEMBL3764354CHEMBL3765189CHEMBL3763698CHEMBL3765499... |
| O04417 | Calcium dependent protein kinase | Zea mays | OrthoMCL BLAST | CHEMBL610874CHEMBL610876CHEMBL611121 |
| Q16644 | MAP kinase-activated protein kinase 3 | Homo sapiens | OrthoMCL BLAST | Pyrrolopyridine, 9Pyrrolopyridine, 16Pyrrolopyridine, 23US11370770, Compound 6j... |
| Q8IBS5 | Calcium-dependent protein kinase 4 | Plasmodium falciparum (isolate 3D7) | OrthoMCL BLAST | CHEMBL2069957CHEMBL2070050CHEMBL2070050CHEMBL2070081 |
| Q8IW41 | MAP kinase-activated protein kinase 5 | Homo sapiens | OrthoMCL BLAST | Pyrrolopyridinone, 1Pyrrolopyridine, 9Pyrrolopyridine, 16Pyrrolopyridine, 23... |
Ortholog Group (OrthoMCL): OG6_100153
Most Similar Human Ortholog: Not available
TM-align score: 0.00 | RMSD: 0.00
Seq Identity: 0.00 | Length: / 513
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130822 | pregnancy up-regulated nonubiquitous CaM kinase |
| ENSG00000134072 | calcium/calmodulin dependent protein kinase I |
| ENSG00000152495 | calcium/calmodulin dependent protein kinase IV |
| ENSG00000183049 | calcium/calmodulin dependent protein kinase ID |
Protein Length: 513 | Molecular Weight (kDa): 59.048
UniProt ID(s): None
PDB ID(s): None
Isoelectric Point: 7.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR002048 | EF-hand domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR011992 | EF-hand domain pair |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| InterPro | IPR018247 | EF-Hand 1, calcium-binding site |
| PFam | PF00069 | Protein kinase domain |
| PFam | PF13499 | EF-hand domain |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 18 | 11 | 20 |
| disruptive | 0 | 10 | 6 | 30 |
| missense | 0 | 5 | 6 | 22 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 38 | 18 | 31 |
| disruptive | 9 | 46 | 24 | 100 |
| missense | 0 | 27 | 19 | 61 |
PlasmoDB Total SNPs: 188
Non-coding: 173 | Synonymous: 15 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9247932 | Molecular cloning and characterization of a second calcium-dependent proteinkinase of Plasmodium falciparum. | Farber PM, Graeser R, Franklin RM, Kappes B | 10.1016/s0166-6851(97)00052-2 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28851850 | Toxoplasma gondii Requires Glycogen Phosphorylase for Balancing AmylopectinStorage and for Efficient Production of Brain Cysts | Sugi T, Tu V, ..., Weiss LM | 10.1128/mBio.01289-17 |
| 11154702 | Toxoplasma gondii attachment to host cells is regulated by a calmodulin-likedomain protein kinase | Kieschnick H, Wakefield T, Narducci CA, Beckers C | 10.1074/jbc.M011045200 |
| 29669003 | Live Attenuated Pru:Deltacdpk2 Strain of Toxoplasma gondii Protects Against Acute,Chronic, and Congenital Toxoplasmosis | Wang JL, Li TT, ..., Zhu XQ | 10.1093/infdis/jiy211 |
| 31399001 | Perspective on current and emerging drugs in the treatment of acute and chronictoxoplasmosis | Lapinskas PJ, Ben-Harari RR | 10.1080/00325481.2019.1655258 |
| 26651943 | Regulation of Starch Stores by a Ca(2+)-Dependent Protein Kinase Is Essential forViable Cyst Development in Toxoplasma gondii | Uboldi AD, McCoy JM, ..., Tonkin CJ | 10.1016/j.chom.2015.11.004 |
| 23792132 | Expression, purification and biochemical characterization of recombinantCa-dependent protein kinase 2 of the malaria parasite Plasmodium falciparum | Lauciello L, Kappes B, Scapozza L, Perozzo R | 10.1016/j.pep.2013.06.006 |
| 34129780 | Role of amylopectin synthesis in Toxoplasma gondii and its implication in vaccinedevelopment against toxoplasmosis | Lyu C, Yang X, ..., Shen B | 10.1098/rsob.200384 |
| 25966270 | Low-level sequence variation in Toxoplasma gondii calcium-dependent proteinkinases among different genotypes | Wang JL, Zhang NZ, ..., Zhu XQ | 10.4238/2015.May.11.28 |