Product Description: transketolase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.2.1.1 (Transketolase);2.2.1.7 (1-deoxy-D-xylulose-5-phosphate synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0004802 | transketolase activity |
| Process | GO:0006098 | pentose-phosphate shunt |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.76 | 0.64 |
| Trophozoite | expressed | 0.62 | 0.65 |
| Schizont | expressed | 0.20 | 0.87 |
| Gametocyte | expressed | 0.42 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0610800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.381 | MFS: -2.533 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0109100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSV3
"Best" AlphaFill ligand hit: MLT ((2r)-2-hydroxybutanedioic acid, Local RMSD=0.08) with 6YAK (Global RMSD=2.70)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.2.1.1 | transketolase | BRENDA SOAP query unsuccessful |
| 2.2.1.7 | 1-deoxy-D-xylulose-5-phosphate synthase | EDTApyruvateketoclomazoneFluoropyruvatenitrosobenzeneD-glyceraldehyde3-Fluoropyruvatebeta-fluoropyruvatebeta-Glycerophosphatebutylacetylphosphonateisopentenyl diphosphateMethylacetylphosphonate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P29401 | Transketolase | Homo sapiens | OMA, OrthoMCL BLAST | CHEMBL252845CHEMBL440237CHEMBL402329CHEMBL403365... |
Ortholog Group (OrthoMCL): OG6_100329
Most Similar Human Ortholog: P29401
TM-align score: 0.92 | RMSD: 2.35
Seq Identity: 0.24 | Length: 602 / 672
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000007350 | transketolase like 1 |
| ENSG00000151005 | transketolase like 2 |
| ENSG00000163931 | transketolase |
Protein Length: 672 | Molecular Weight (kDa): 75.815
UniProt ID(s): C6KSV3
PDB ID(s): None
Isoelectric Point: 6.95
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005474 | Transketolase, N-terminal |
| InterPro | IPR005475 | Transketolase-like, pyrimidine-binding domain |
| InterPro | IPR005478 | Transketolase, bacterial-like |
| InterPro | IPR009014 | Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II |
| InterPro | IPR020826 | Transketolase binding site |
| InterPro | IPR029061 | Thiamin diphosphate-binding fold |
| InterPro | IPR033247 | Transketolase family |
| PFam | PF00456 | Transketolase, N-terminal |
| PFam | PF02779 | Transketolase-like, pyrimidine-binding domain |
| Superfamily | SSF52518 | Thiamin diphosphate-binding fold |
| Superfamily | SSF52922 | Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 26 | 18 | 37 |
| disruptive | 2 | 19 | 25 | 42 |
| missense | 2 | 18 | 25 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 59 | 24 | 39 |
| disruptive | 8 | 61 | 37 | 87 |
| missense | 4 | 53 | 34 | 63 |
PlasmoDB Total SNPs: 74
Non-coding: 56 | Synonymous: 13 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18456347 | Molecular cloning and characterization of Plasmodium falciparum transketolase. | Joshi S, Singh AR, ..., Saxena JK | 10.1016/j.molbiopara.2008.03.005 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |