Product Description: transcription elongation factor SPT5, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0032044 | DSIF complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0006357 | regulation of transcription by RNA polymerase II |
| Process | GO:0006368 | transcription elongation from RNA polymerase II promoter |
| Process | GO:0006351 | transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.62 | 0.35 |
| Trophozoite | expressed | 1.18 | 0.41 |
| Schizont | expressed | 0.54 | 0.69 |
| Gametocyte | expressed | 1.26 | 0.39 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0610900
Resistome Missense Mutations: Q506K
Resistome Compounds with Missense Mutations: Halofuginone
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.128 | MFS: -2.889 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0109200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0109200)
Modification: Disrupted | RMgm-1651
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSV4
"Best" AlphaFill ligand hit: 017 ((3r,3as,6ar)-hexahydrofuro[2,3-b]furan-3-yl(1s,2r)-3-[[(4-aminophenyl)sulfonyl](isobutyl)amino]-1-benzyl-2-hydroxypropylcarbamate, Local RMSD=6.63) with 3OGP (Global RMSD=19.57)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101219
Most Similar Human Ortholog: M0R105
TM-align score: 0.45 | RMSD: 3.38
Seq Identity: 0.04 | Length: 50 / 1280
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000196235 | SPT5 homolog, DSIF elongation factor subunit |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 34 | 26 | 69 |
| disruptive | 24 | 104 | 40 | 147 |
| missense | 9 | 73 | 30 | 123 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 87 | 47 | 60 |
| disruptive | 54 | 197 | 96 | 216 |
| missense | 15 | 139 | 78 | 162 |
PlasmoDB Total SNPs: 150
Non-coding: 71 | Synonymous: 58 | Nonsynonymous: 20 | Stop Codon: 1
Protein Length: 1280 | Molecular Weight (kDa): 149.366
UniProt ID(s): C6KSV4
PDB ID(s): None
Isoelectric Point: 6.77
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005100 | NGN domain |
| InterPro | IPR005824 | KOW |
| InterPro | IPR005825 | Ribosomal protein L24/L26, conserved site |
| InterPro | IPR008991 | Translation protein SH3-like domain superfamily |
| InterPro | IPR039385 | NGN domain, eukaryotic |
| InterPro | IPR039659 | Transcription elongation factor SPT5 |
| InterPro | IPR041973 | Spt5, KOW domain repeat 1 |
| PFam | PF03439 | NGN domain |
| Superfamily | SSF50104 | Translation protein SH3-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |