Product Description: cytoplasmic tRNA 2-thiolation protein 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.- (Nucleotidyltransferases.);2.7.7.72 (CCA tRNA nucleotidyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0002144 | cytosolic tRNA wobble base thiouridylase complex |
| Function | GO:0000049 | tRNA binding |
| Process | GO:0032447 | protein urmylation |
| Process | GO:0002143 | tRNA wobble position uridine thiolation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.36 | 0.79 |
| Schizont | expressed | 0.46 | 0.72 |
| Gametocyte | expressed | 0.30 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0612600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.971 | MFS: -2.37 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSW9
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.36) with 5B4E (Global RMSD=2.26)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.- | Ex: 2-hydroxyethylphosphonate cytidylyltransferase | ADPAMPUTPKClNAD+NADP+NaAD+ppGpppppGppsuraminADPribosecisplatin... |
| 2.7.7.72 | CCA tRNA nucleotidyltransferase | Neocuproine2,2-dipyridylethylnitrosourea2,2,2-Terpyridyl1,7-phenanthroline1,10-phenanthrolinebathophenanthroline |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100947
Most Similar Human Ortholog: Q7Z7A3
TM-align score: 0.86 | RMSD: 1.77
Seq Identity: 0.40 | Length: 314 / 482
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000142544 | cytosolic thiouridylase subunit 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 18 | 15 | 27 |
| disruptive | 13 | 55 | 27 | 75 |
| missense | 4 | 42 | 20 | 59 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 43 | 16 | 37 |
| disruptive | 28 | 131 | 59 | 145 |
| missense | 12 | 84 | 43 | 91 |
PlasmoDB Total SNPs: 154
Non-coding: 95 | Synonymous: 32 | Nonsynonymous: 27 | Stop Codon: 0
Protein Length: 482 | Molecular Weight (kDa): 55.955
UniProt ID(s): C6KSW9
PDB ID(s): None
Isoelectric Point: 6.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000541 | Cytoplasmic tRNA 2-thiolation protein 1 |
| InterPro | IPR011063 | tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal |
| InterPro | IPR020554 | Uncharacterised protein family UPF0021, conserved site |
| InterPro | IPR032442 | Cytoplasmic tRNA 2-thiolation protein 1, C-terminal |
| PFam | PF01171 | tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal |
| PFam | PF16503 | Cytoplasmic tRNA 2-thiolation protein 1, C-terminal |
| Superfamily | SSF52402 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 14551258 | Urmylation: a ubiquitin-like pathway that functions during invasive growth andbudding in yeast | Goehring AS, Rivers DM, Sprague GF Jr | 10.1091/mbc.e03-02-0079 |
| 25747122 | Loss of anticodon wobble uridine modifications affects tRNA(Lys) function andprotein levels in Saccharomyces cerevisiae | Klassen R, Grunewald P, ..., Schaffrath R | 10.1371/journal.pone.0119261 |