Product Description: 6-cysteine protein P12
SignalP Peptide: MIKLSKKYCLGISFVLYILLSVCEG
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0046658 | anchored component of plasma membrane |
| Component | GO:0045177 | apical part of cell |
| Component | GO:0009986 | cell surface |
| Component | GO:0020008 | rhoptry |
| Component | GO:0020004 | symbiont-containing vacuolar space |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.19 | 0.90 |
| Trophozoite | expressed | 0.46 | 0.74 |
| Schizont | expressed | 2.06 | 0.15 |
| Gametocyte | expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0612700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.155 | MFS: -3.277 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1107600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: P19259
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 5 | 12 | 19 |
| disruptive | 9 | 21 | 16 | 64 |
| missense | 9 | 21 | 16 | 55 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 23 | 17 | 31 |
| disruptive | 15 | 78 | 39 | 120 |
| missense | 14 | 68 | 37 | 89 |
PlasmoDB Total SNPs: 213
Non-coding: 185 | Synonymous: 18 | Nonsynonymous: 9 | Stop Codon: 1
Protein Length: 347 | Molecular Weight (kDa): 39.434
UniProt ID(s): C5HE14, C5HE15, C5HE16, C5HE17, C5HE18, C5HE20, C5HE21, C5HE22, C5HE23, C5HE26, C5HE27, C6KSX0, P19259, Q9NGS1
PDB ID(s): 2LOE, 2YMO, 7S7Q, 7S7R
Isoelectric Point: 8.41
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010884 | 6-Cysteine (6-Cys) domain |
| PFam | PF07422 | 6-Cysteine (6-Cys) domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16203726 | Distinct protein classes including novel merozoite surface antigens in Raft-likemembranes of Plasmodium falciparum. | Sanders PR, Gilson PR, ..., Crabb BS | 10.1074/jbc.M509631200 |
| 22848665 | Biochemical and functional analysis of two Plasmodium falciparum blood-stage6-cys proteins: P12 and P41. | Taechalertpaisarn T, Crosnier C, ..., Crabb BS | 10.1371/journal.pone.0041937 |
| 23511632 | Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12)reveals a unique interdomain organization and the potential for an antiparallelarrangement with Pf41. | Tonkin ML, Arredondo SA, ..., Boulanger MJ | 10.1074/jbc.M113.455667 |
| 28202027 | A novel Pfs38 protein complex on the surface of Plasmodium falciparum blood-stagemerozoites. | Paul G, Deshmukh A, ..., Malhotra P | 10.1186/s12936-017-1716-0 |
| 33480416 | Nanobody generation and structural characterization of Plasmodium falciparum6-cysteine protein Pf12p. | Dietrich MH, Chan LJ, ..., Tham WH | 10.1042/BCJ20200415 |