PF3D7_0612900 (NOG1)

Genome location: Pf3D7_06_v3:530,674..533,646(+)

Genome classification: Core

Function and Localization

Product Description: nucleolar GTP-binding protein 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Component	GO:0030687	preribosome, large subunit precursor
Function	GO:0005525	GTP binding
Process	GO:0042273	ribosomal large subunit biogenesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.39	0.81
Trophozoite	expressed	0.44	0.74
Schizont	expressed	0.09	0.95
Gametocyte	expressed	0.42	0.76

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0612900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.836 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0111200)

Relative Growth Rate: 0.27 ± 0.21
Confidence: 4.52

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSX2

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.40) with 2QU8 (Global RMSD=5.25)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101289

Most Similar Human Ortholog: Q5T3R7

TM-align score: 0.97 | RMSD: 0.80

Seq Identity: 0.57 | Length: 140 / 686

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000107937	GTP binding protein 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	21	34	25	49
disruptive	9	45	27	83
missense	7	44	26	77

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	82	28	34
disruptive	21	122	73	160
missense	17	105	61	117

PlasmoDB Total SNPs: 171

Non-coding: 120 | Synonymous: 35 | Nonsynonymous: 13 | Stop Codon: 3

Protein Information

Protein Length: 686 | Molecular Weight (kDa): 79.534

UniProt ID(s): C6KSX2

PDB ID(s): 2QU8

Isoelectric Point: 10.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006073	GTP binding domain
InterPro	IPR010674	Nucleolar GTP-binding protein 1, Rossman-fold domain
InterPro	IPR012973	NOG, C-terminal
InterPro	IPR024926	Nucleolar GTP-binding protein 1
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR041623	NOG1, N-terminal helical domain
PFam	PF06858	Nucleolar GTP-binding protein 1, Rossman-fold domain
PFam	PF08155	NOG, C-terminal
PFam	PF17835	NOG1, N-terminal helical domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104