About Gene List

PF3D7_0613300 (ROP14)

Genome location: Pf3D7_06_v3:539,725..545,452(-)

Genome classification: Core

Function and Localization

Product Description: rhoptry protein ROP14

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005789 endoplasmic reticulum membrane
Component GO:0020008 rhoptry
Process GO:0051604 protein maturation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.03 0.98
Trophozoite not expressed 0.05 0.97
Schizont expressed 1.39 0.43
Gametocyte not expressed 0.09 0.95

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0613300

Resistome Missense Mutations: F290V

Resistome Compounds with Missense Mutations: Suloctidil

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.535 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0111600)

  • Relative Growth Rate: 1.01 ± 0.10
  • Confidence: 6.07

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0111600)

Modification: Disrupted | RMgm-1663

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSX6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106580

Most Similar Human Ortholog: H3BN37

TM-align score: 0.90 | RMSD: 1.64

Seq Identity: 0.22 | Length: 194 / 1422

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000103227 lipase maturation factor 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 8 35 37 80
disruptive 38 149 93 224
missense 36 145 90 207

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 13 92 60 105
disruptive 73 314 175 313
missense 54 268 158 249

PlasmoDB Total SNPs: 191

Non-coding: 106 | Synonymous: 48 | Nonsynonymous: 37 | Stop Codon: 0

Protein Information

Protein Length: 1422 | Molecular Weight (kDa): 172.015

UniProt ID(s): C6KSX6

PDB ID(s): None

Isoelectric Point: 9.23

Protein Domain Annotations:

Source Family ID Description
InterPro IPR009613 Lipase maturation factor
PFam PF06762 Lipase maturation factor
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23049965 Subcompartmentalisation of proteins in the rhoptries correlates with orderedevents of erythrocyte invasion by the blood stage malaria parasite. Zuccala ES, Gout AM, ..., Baum J 10.1371/journal.pone.0046160
35953918 Development of an Immunochromatographic Test Based on Rhoptry Protein 14 forSerological Detection of Toxoplasma gondii Infection in Swine Yang Y, Huang Y, ..., Du A 10.3390/ani12151929