PF3D7_0613900 (MyoE)

Genome location: Pf3D7_06_v3:579,250..586,589(-)

Genome classification: Core

Function and Localization

Product Description: myosin E, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0015629	actin cytoskeleton
Component	GO:0045178	basal part of cell
Component	GO:0005737	cytoplasm
Component	GO:0005829	cytosol
Component	GO:0016459	myosin complex
Component	GO:0005634	nucleus
Component	GO:0031982	vesicle
Function	GO:0005524	ATP binding
Function	GO:0003779	actin binding
Function	GO:0051015	actin filament binding
Function	GO:0030898	actin-dependent ATPase activity
Function	GO:0000146	microfilament motor activity
Process	GO:0007015	actin filament organization
Process	GO:0048870	cell motility
Process	GO:0030050	vesicle transport along actin filament

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.38	0.85
Trophozoite	possibly expressed	0.18	0.89
Schizont	expressed	1.73	0.47
Gametocyte	not expressed	0.17	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0613900

Resistome Missense Mutations: L1869V

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.184 | #Insertions: 12

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0112200)

Modification: Tagged | RMgm-4665

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSY1

"Best" AlphaFill ligand hit: P15 (2,5,8,11,14,17-hexaoxanonadecan-19-ol, Local RMSD=0.12) with 6QDJ (Global RMSD=2.73)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P08799	Myosin-2 heavy chain		OMA	CHEBI:75379CHEMBL4165503CHEMBL4166562CHEMBL4162111
P35579	Myosin-9		OMA	CHEBI:75379

Orthology Information

Ortholog Group (OrthoMCL): OG6_533718

No human ortholog(s)

Protein Information

Protein Length: 2153 | Molecular Weight (kDa): 255.084

UniProt ID(s): C6KSY1

PDB ID(s): None

Isoelectric Point: 4.5

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001609	Myosin head, motor domain
InterPro	IPR025662	Sigma-54 interaction domain, ATP-binding site 1
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00063	Myosin head, motor domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	85	45	109
disruptive	87	269	141	338
missense	53	214	123	282

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	34	155	59	128
disruptive	144	493	208	465
missense	90	390	175	352

PlasmoDB Total SNPs: 395

Non-coding: 90 | Synonymous: 173 | Nonsynonymous: 132 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
31283102	Systematic analysis of Plasmodium myosins reveals differential expression,localisation, and function in invasive and proliferative parasite stages.	Wall RJ, Zeeshan M, ..., Tewari R	10.1111/cmi.13082
32554712	The Functional Characterization of TcMyoF Implicates a Family ofCytostome-Cytopharynx Targeted Myosins as Integral to the Endocytic Machinery ofTrypanosoma cruzi	Chasen NM, Etheridge MG, Etheridge RD	10.1128/mSphere.00313-20