PF3D7_0614000

Genome location: Pf3D7_06_v3:586,596..589,075(-)

Genome classification: Core

Function and Localization

Product Description: thiamine-phosphate synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.5.1.3 (Thiamine phosphate synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0004789	thiamine-phosphate diphosphorylase activity
Process	GO:0009228	thiamine biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.39	0.84
Trophozoite	expressed	0.66	0.63
Schizont	expressed	0.52	0.71
Gametocyte	expressed	0.38	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0614000

Resistome Missense Mutations: L101F

Resistome Compounds with Missense Mutations: MMV019313

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.228 | #Insertions: 8

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSY2

"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.65) with 3NM1 (Global RMSD=4.00)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.5.1.3	thiamine phosphate synthase	ATPADPGTPCTPUTPPCMBthiamindiphosphatephosphocreatineacetyl phosphatethiamin phosphatephosphoenolpyruvate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102838

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	20	12	27
disruptive	7	37	24	75
missense	7	37	23	70

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	36	23	36
disruptive	13	98	46	120
missense	8	88	40	94

PlasmoDB Total SNPs: 133

Non-coding: 112 | Synonymous: 8 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 538 | Molecular Weight (kDa): 62.651

UniProt ID(s): C6KSY2, Q3MSL9

PDB ID(s): None

Isoelectric Point: 8.22

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR022998	Thiamine phosphate synthase/TenI
InterPro	IPR036206	Thiamin phosphate synthase superfamily
PFam	PF02581	Thiamine phosphate synthase/TenI
Superfamily	SSF51391	Thiamin phosphate synthase superfamily