PF3D7_0614400 (CWF7)

Genome location: Pf3D7_06_v3:602,024..605,863(+)

Genome classification: Core

Function and Localization

Product Description: pre-mRNA-splicing factor CWF7, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000974	Prp19 complex
Component	GO:0071013	catalytic step 2 spliceosome
Process	GO:0006397	mRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.25	0.88
Trophozoite	expressed	0.24	0.85
Schizont	possibly expressed	0.12	0.92
Gametocyte	expressed	0.27	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0614400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.199 | MFS: -3.203 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSY5

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103435

Most Similar Human Ortholog: O75934

TM-align score: 0.82 | RMSD: 2.38

Seq Identity: 0.21 | Length: 207 / 255

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000116752	BCAS2 pre-mRNA processing factor

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	15	7	21
disruptive	3	18	20	38
missense	3	17	18	37

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	32	11	27
disruptive	9	54	46	99
missense	6	46	36	52

PlasmoDB Total SNPs: 198

Non-coding: 175 | Synonymous: 18 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 255 | Molecular Weight (kDa): 30.85

UniProt ID(s): C6KSY5

PDB ID(s): None

Isoelectric Point: 5.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008409	Pre-mRNA-splicing factor SPF27
PFam	PF05700	Pre-mRNA-splicing factor SPF27
Superfamily	N/A	N/A