Product Description: enoyl-acyl carrier reductase
SignalP Peptide: MNKISQRLLFLFLHFYTIVCF
# Transmembrane Domains: 0
EC Numbers: 1.3.1.9 (Enoyl-[acyl-carrier-protein] reductase (NADH))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0016631 | enoyl-[acyl-carrier-protein] reductase activity |
| Process | GO:0006633 | fatty acid biosynthetic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 1.00 |
| Trophozoite | not expressed | 0.01 | 1.00 |
| Schizont | not expressed | 0.01 | 1.00 |
| Gametocyte | expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0615100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.505 | MFS: -2.462 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1229800)
Modification: Disrupted | RMgm-256
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSZ2
"Best" AlphaFill ligand hit: 2NV (jeffamine ed-2001, Local RMSD=0.04) with 4OXN (Global RMSD=2.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.3.1.9 | enoyl-[acyl-carrier-protein] reductase (NADH) | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q6UCJ9 | Enoyl-acyl carrier reductase ENR | Toxoplasma gondii | OMA, OrthoMCL BLAST | TriclosanCHEMBL200379CHEMBL200585CHEMBL200492... |
| P9WGR1 | Enoyl-[acyl-carrier-protein] reductase [NADH] | Mycobacterium tuberculosis | OrthoMCL, OrthoMCL BLAST | Pyrrolidine Carboxamide Compound s4Pyrrolidine Carboxamide Compound s6Pyrrolidine Carboxamide Compound s11Pyrrolidine Carboxamide Compound s15... |
| P0AEK4 | Enoyl-[acyl-carrier-protein] reductase [NADH] FabI | Escherichia coli | OrthoMCL, OrthoMCL BLAST | indole naphthyridinone 29indole naphthyridinone 30indole naphthyridinone 31indole naphthyridinone 32... |
| Q5NGQ3 | Enoyl-[acyl-carrier-protein] reductase [NADH] | OrthoMCL, OrthoMCL BLAST | CHEMBL1945507CHEMBL1341978CHEMBL1341978CHEMBL2398664... | |
| P54616 | Enoyl-[acyl-carrier-protein] reductase [NADH] FabI | Bacillus subtilis | OrthoMCL, OrthoMCL BLAST | CHEMBL260770CHEMBL260771CHEMBL261109CHEMBL407894... |
| Q9BJJ9 | Enoyl-ACP reductase | Plasmodium falciparum | OrthoMCL BLAST | TriclosanTriclosan derivative, 2Triclosan derivative, 3Triclosan derivative, 4... |
| A0Q794 | Enoyl-[acyl-carrier-protein] reductase [NADH] | Francisella tularensis | OrthoMCL BLAST | TriclosanPT51PT55PT52... |
| Q965D5 | Enoyl-acyl-carrier protein reductase | Plasmodium falciparum | OrthoMCL BLAST | CHEMBL197322CHEMBL198831CHEMBL197474CHEMBL198886... |
| Q2FZQ3 | Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI | Staphylococcus aureus | OrthoMCL BLAST | US8623865, 1US8623865, 2US8623865, 3US8846711, 2... |
| Q6GI75 | Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI | Staphylococcus aureus | OrthoMCL BLAST | 1,4-Benzodiazepine deriv. 2benzamide deriv. 5aminopyridine deriv. 9aminopyrimidine deriv. 10... |
| Q3JG55 | Enoyl-[acyl-carrier-protein] reductase [NADH] | OrthoMCL BLAST | TriclosanPT443MUT056399AIDS227443... | |
| Q9BH77 | Enoyl-acyl carrier reductase | Plasmodium falciparum | OrthoMCL BLAST | TriclosanPhenylaminoacetic acid benzylidene hydrazine analog, 7Phenylaminoacetic acid benzylidene hydrazine analog, 17 |
| P44432 | Enoyl-[acyl-carrier-protein] reductase [NADH] FabI | Haemophilus influenzae | OrthoMCL BLAST | 1,4-Benzodiazepine deriv. 11,4-Benzodiazepine deriv. 21,4-Benzodiazepine deriv. 4benzamide deriv. 5... |
Protein Length: 432 | Molecular Weight (kDa): 49.762
UniProt ID(s): C6KSZ2, Q965D5, Q9BH77, Q9BJJ9
Isoelectric Point: 9.48
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR014358 | Enoyl-[acyl-carrier-protein] reductase (NADH) |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF13561 | N/A |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 20 | 16 | 23 |
| disruptive | 11 | 42 | 24 | 59 |
| missense | 11 | 40 | 24 | 46 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 38 | 30 | 23 |
| disruptive | 17 | 96 | 54 | 101 |
| missense | 15 | 85 | 45 | 69 |
PlasmoDB Total SNPs: 70
Non-coding: 39 | Synonymous: 19 | Nonsynonymous: 12 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11175846 | Triclosan offers protection against blood stages of malaria by inhibitingenoyl-ACP reductase of Plasmodium falciparum. | Surolia N, Surolia A | 10.1038/84612 |
| 11792710 | Structural elucidation of the specificity of the antibacterial agent triclosanfor malarial enoyl acyl carrier protein reductase. | Perozzo R, Kuo M, ..., Sacchettini JC | 10.1074/jbc.M112000200 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 18424102 | Inhibiting enoyl-ACP reductase (FabI) across pathogenic microorganisms by linearsesquiterpene lactones from Anthemis auriculata. | Karioti A, Skaltsa H, ..., Tasdemir D | 10.1016/j.phymed.2008.02.018 |
| 19064257 | The fatty acid biosynthesis enzyme FabI plays a key role in the development ofliver-stage malarial parasites. | Yu M, Kumar TR, ..., Fidock DA | 10.1016/j.chom.2008.11.001 |
| 28820936 | Elucidating Substrate Promiscuity within the FabI Enzyme Family. | Freund GS, O'Brien TE, ..., Siegel JB | 10.1021/acschembio.7b00400 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |