Product Description: cdc2-related protein kinase 5
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.22 (Cyclin-dependent kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0016592 | mediator complex |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:0048471 | perinuclear region of cytoplasm |
| Function | GO:0008353 | RNA polymerase II CTD heptapeptide repeat kinase activity |
| Function | GO:0004693 | cyclin-dependent protein serine/threonine kinase activity |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0051726 | regulation of cell cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.37 | 0.78 |
| Schizont | expressed | 0.33 | 0.81 |
| Gametocyte | expressed | 0.54 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0615500
Resistome Missense Mutations: N330D
Resistome Compounds with Missense Mutations: AN13762, DSM1, Halofuginone
Resistome # Samples with Disruptive Mutations: 28 (28 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.133 | MFS: -2.786 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1230200)
Modification: Tagged | RMgm-4848
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KSZ6
"Best" AlphaFill ligand hit: HVK (pyridin-2-amine, Local RMSD=0.07) with 6QA1 (Global RMSD=5.01)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.22 | cyclin-dependent kinase | AMPDRBp16p18p21p27NMDATHZ1ICK1ICK2ICK3ICK4... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 30 | 14 | 60 |
| disruptive | 39 | 46 | 24 | 75 |
| missense | 11 | 20 | 15 | 52 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 85 | 34 | 50 |
| disruptive | 60 | 129 | 63 | 172 |
| missense | 17 | 64 | 49 | 101 |
PlasmoDB Total SNPs: 208
Non-coding: 162 | Synonymous: 27 | Nonsynonymous: 19 | Stop Codon: 0
Protein Length: 705 | Molecular Weight (kDa): 82.948
UniProt ID(s): C6KSZ6
PDB ID(s): None
Isoelectric Point: 8.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 33608523 | 20S proteasomes secreted by the malaria parasite promote its growth. | Dekel E, Yaffe D, ..., Regev-Rudzki N | 10.1038/s41467-021-21344-8 |
| 14756307 | Sensitization of defense responses and activation of programmed cell death by apathogen-induced receptor-like protein kinase in Arabidopsis | Chen K, Du L, Chen Z | 10.1023/B:PLAN.0000009265.72567.58 |
| 28830940 | The Toxoplasma Centrocone Houses Cell Cycle Regulatory Factors | Naumov A, Kratzer S, ..., White MW | 10.1128/mBio.00579-17 |
| 29141230 | Sub-minute Phosphoregulation of Cell Cycle Systems during Plasmodium GameteFormation | Invergo BM, Brochet M, ..., Billker O | 10.1016/j.celrep.2017.10.071 |
| 32568069 | A divergent cyclin/cyclin-dependent kinase complex controls the atypicalreplication of a malaria parasite during gametogony and transmission | Balestra AC, Zeeshan M, ..., Brochet M | 10.7554/eLife.56474 |
| 36154191 | Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection ofthe Mosquito | Kumar S, Gargaro OR, Kappe SHI | 10.1128/mbio.02227-22 |
| 31817249 | CRK5 Protein Kinase Contributes to the Progression of Embryogenesis ofArabidopsis thaliana | Baba AI, Valkai I, ..., Cseplo A | 10.3390/ijms20246120 |
| 22047078 | Structural and evolutionary divergence of eukaryotic protein kinases inApicomplexa | Talevich E, Mirza A, Kannan N | 10.1186/1471-2148-11-321 |
| 27406784 | Overexpression of an Arabidopsis cysteine-rich receptor-like protein kinase,CRK5, enhances abscisic acid sensitivity and confers drought tolerance | Lu K, Liang S, ..., Zhang DP | 10.1093/jxb/erw266 |