PF3D7_0615700 (ALYREF)

Genome location: Pf3D7_06_v3:656,020..657,929(+)

Genome classification: Core

Function and Localization

Product Description: THO complex subunit 4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003723	RNA binding
Process	GO:0006406	mRNA export from nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.15	0.93
Trophozoite	not expressed	0.31	0.81
Schizont	expressed	0.14	0.90
Gametocyte	possibly expressed	0.24	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0615700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 1 | MFS: -0.987 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1230500)

Modification: Disrupted | RMgm-5087

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KSZ8

"Best" AlphaFill ligand hit: M2M (1-methoxy-2-(2-methoxyethoxy)ethane, Local RMSD=1.29) with 4ED5 (Global RMSD=8.38)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101458

Most Similar Human Ortholog: E9PB61

TM-align score: 0.53 | RMSD: 2.74

Seq Identity: 0.28 | Length: 95 / 156

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000183684	Aly/REF export factor

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	10	6	9
disruptive	2	7	8	13
missense	2	7	8	9

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	18	11	7
disruptive	2	23	14	50
missense	2	17	10	31

PlasmoDB Total SNPs: 177

Non-coding: 172 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 1

Protein Information

Protein Length: 156 | Molecular Weight (kDa): 18.853

UniProt ID(s): C6KSZ8

PDB ID(s): None

Isoelectric Point: 9.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR035979	RNA-binding domain superfamily
PFam	PF00076	RNA recognition motif domain
Superfamily	SSF54928	RNA-binding domain superfamily