Product Description: pyridoxal kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.35 (Pyridoxal kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0005524 | ATP binding |
| Function | GO:0070280 | pyridoxal binding |
| Function | GO:0008478 | pyridoxal kinase activity |
| Function | GO:0070281 | pyridoxamine binding |
| Function | GO:0070282 | pyridoxine binding |
| Process | GO:0016310 | phosphorylation |
| Process | GO:0009443 | pyridoxal 5'-phosphate salvage |
| Process | GO:0008614 | pyridoxine metabolic process |
| Process | GO:0000304 | response to singlet oxygen |
| Process | GO:0042819 | vitamin B6 biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.18 | 0.89 |
| Schizont | possibly expressed | 0.04 | 0.97 |
| Gametocyte | not expressed | 0.19 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0616000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.478 | MFS: -2.377 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT01
"Best" AlphaFill ligand hit: PXL (3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde, Local RMSD=0.22) with 6SU9 (Global RMSD=0.81)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.35 | pyridoxal kinase | ADPAMPNADHOximeazineZnATP2-sperminedopaminetyramineCaffeinelevodopaserotonin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P01042 | Kininogen-1 | Homo sapiens | OrthoMCL BLAST | US8592426, 1US8592426, 2US8592426, 3US8592426, 4... |
Ortholog Group (OrthoMCL): OG6_102185
Most Similar Human Ortholog: O00764
TM-align score: 0.84 | RMSD: 2.06
Seq Identity: 0.30 | Length: 282 / 497
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000160209 | pyridoxal kinase |
Protein Length: 497 | Molecular Weight (kDa): 57.189
UniProt ID(s): C6KT01
PDB ID(s): 6SU9
Isoelectric Point: 7.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004625 | Pyridoxine kinase |
| InterPro | IPR013749 | Pyridoxamine kinase/Phosphomethylpyrimidine kinase |
| InterPro | IPR029056 | Ribokinase-like |
| PFam | PF08543 | Pyridoxamine kinase/Phosphomethylpyrimidine kinase |
| Superfamily | SSF53613 | Ribokinase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 19 | 7 | 16 |
| disruptive | 45 | 41 | 22 | 66 |
| missense | 25 | 25 | 15 | 54 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 28 | 8 | 27 |
| disruptive | 57 | 82 | 37 | 107 |
| missense | 31 | 53 | 27 | 64 |
PlasmoDB Total SNPs: 253
Non-coding: 103 | Synonymous: 68 | Nonsynonymous: 81 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15590634 | Analysis of the vitamin B6 biosynthesis pathway in the human malaria parasitePlasmodium falciparum. | Wrenger C, Eschbach ML, ..., Walter RD | 10.1074/jbc.M412475200 |
| 25372829 | Purification, crystallization and preliminary X-ray diffraction analysis ofpyridoxal kinase from Plasmodium falciparum (PfPdxK). | Kronenberger T, Lunev S, Wrenger C, Groves MR | 10.1107/S2053230X14019864 |
| 35598242 | Pyridoxal Kinase of Disease-causing Human Parasites: Structural and FunctionalInsights to Understand its Role in Drug Discovery | Saini M, Are S, Qureshi IA | 10.2174/1389203723666220519155025 |
| 37937770 | Cheminformatics-based discovery of new organoselenium compounds with potentialfor the treatment of cutaneous and visceral leishmaniasis | Ugbe FA, Shallangwa GA, ..., Abdalla M | 10.1080/07391102.2023.2279269 |
| 38403043 | Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani | Roy PK, Paul A, ..., Singh S | 10.1016/j.biochi.2024.02.009 |
| 38150934 | Discovery and characterization of natural product luteolin as an effectiveinhibitor of human pyridoxal kinase | Zhu Y, Bao G, ..., Dang Y | 10.1016/j.bioorg.2023.107057 |