Product Description: kinetochore protein NDC80
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031262 | Ndc80 complex |
| Component | GO:0000775 | chromosome, centromeric region |
| Component | GO:0000778 | condensed nuclear chromosome kinetochore |
| Component | GO:0005874 | microtubule |
| Component | GO:0005634 | nucleus |
| Function | GO:0008017 | microtubule binding |
| Process | GO:0051315 | attachment of mitotic spindle microtubules to kinetochore |
| Process | GO:0007059 | chromosome segregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.13 | 0.94 |
| Trophozoite | expressed | 0.90 | 0.53 |
| Schizont | expressed | 0.92 | 0.51 |
| Gametocyte | expressed | 1.22 | 0.40 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0616200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.167 | MFS: -3.302 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1115700)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1115700)
Modification: Tagged | RMgm-5364
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT03
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103226
Most Similar Human Ortholog: I3L0P0
TM-align score: 0.92 | RMSD: 0.43
Seq Identity: 0.09 | Length: 32 / 591
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000080986 | NDC80 kinetochore complex component |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 22 | 19 | 34 |
| disruptive | 5 | 24 | 22 | 68 |
| missense | 5 | 24 | 22 | 58 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 58 | 28 | 32 |
| disruptive | 9 | 77 | 63 | 129 |
| missense | 8 | 64 | 49 | 86 |
PlasmoDB Total SNPs: 116
Non-coding: 86 | Synonymous: 27 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 591 | Molecular Weight (kDa): 69.875
UniProt ID(s): C6KT03
PDB ID(s): None
Isoelectric Point: 5.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005550 | Kinetochore protein Ndc80 |
| PFam | PF03801 | Kinetochore protein Ndc80 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 32049018 | Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and AreEssential for Parasite Proliferation and Transmission. | Pandey R, Abel S, ..., Tewari R | 10.1016/j.celrep.2020.01.033 |
| 32501284 | Real-time dynamics of Plasmodium N DC80 reveals unusual modes of chromosomesegregation during parasite proliferation | Zeeshan M, Pandey R, ..., Tewari R | 10.1242/jcs.245753 |
| 36030238 | Repurposing the mitotic machinery to drive cellular elongation and chromatinreorganisation in Plasmodium falciparum gametocytes. | Li J, Shami GJ, ..., Tilley L | 10.1038/s41467-022-32579-4 |
| 37962556 | Humanization reveals pervasive incompatibility of yeast and human kinetochorecomponents | Olafsson G, Haase MAB, Boeke JD | 10.1093/g3journal/jkad260 |
| 38305688 | Kinetochore-microtubule error correction for biorientation: lessons from yeast | Li S, Kasciukovic T, Tanaka TU | 10.1042/BST20221261 |
| 37950911 | A role for beta-1,6- and beta-1,3-glucans in kinetochore function in Saccharomycescerevisiae | Kshirsagar R, Munhoven A, Tran Nguyen TM, Ehrenhofer-Murray AE | 10.1093/genetics/iyad195 |
| 37986816 | An interaction hub on Ndc80 complex facilitates dynamic recruitment of Mps1 toyeast kinetochores to promote accurate chromosome segregation | Parnell EJ, Jenson E, Miller MP | 10.1101/2023.11.07.566082 |
| 37506202 | Cryo-EM structure of the complete inner kinetochore of the budding yeast pointcentromere | Dendooven T, Zhang Z, ..., Barford D | 10.1126/sciadv.adg7480 |