PF3D7_0616700

Genome location: Pf3D7_06_v3:691,710..694,810(-)

Genome classification: Core

Function and Localization

Product Description: ras GTPAse, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.5.2 (Small monomeric GTPase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005768	endosome
Process	GO:0030100	regulation of endocytosis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.03	0.99
Trophozoite	possibly expressed	0.12	0.93
Schizont	possibly expressed	0.03	0.98
Gametocyte	possibly expressed	0.40	0.76

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0616700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.833 | MFS: -2.447 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1116200)

Relative Growth Rate: 1.01 ± 0.06
Confidence: 7.10

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1116200)

Modification: Disrupted | RMgm-3015

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT08

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.5.2	small monomeric GTPase	GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532442

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	30	19	36
disruptive	17	68	28	71
missense	6	45	24	59

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	56	29	26
disruptive	31	143	51	152
missense	16	97	38	89

PlasmoDB Total SNPs: 140

Non-coding: 94 | Synonymous: 35 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 516 | Molecular Weight (kDa): 61.95

UniProt ID(s): C6KT08

PDB ID(s): None

Isoelectric Point: 7.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001806	Small GTPase
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00071	Small GTPase
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase