Product Description: malate:quinone oxidoreductase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.1.5.4 (Malate dehydrogenase (quinone))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0016020 | membrane |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0030060 | L-malate dehydrogenase activity |
| Function | GO:0008924 | malate dehydrogenase (quinone) activity |
| Function | GO:0016491 | oxidoreductase activity |
| Process | GO:0006090 | pyruvate metabolic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.36 | 0.77 |
| Schizont | expressed | 0.12 | 0.91 |
| Gametocyte | expressed | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0616800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.992 | MFS: -0.442 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1116300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1116300)
Modification: Disrupted | RMgm-3016
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT09
"Best" AlphaFill ligand hit: 9KH (6-carboxy methyl-4-hydroxy-2-pyridinol, Local RMSD=4.26) with 5O4J (Global RMSD=13.70)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.1.5.4 | malate dehydrogenase (quinone) | KClKSCNMgCl2CaCl2CuSO4ZnCl2MnCl2CuCl2CoCl2NiCl2AlCl3NiSO4... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 16 | 12 | 30 |
| disruptive | 6 | 7 | 11 | 25 |
| missense | 6 | 7 | 10 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 41 | 18 | 38 |
| disruptive | 10 | 42 | 31 | 104 |
| missense | 7 | 29 | 25 | 75 |
PlasmoDB Total SNPs: 145
Non-coding: 127 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 2
Protein Length: 521 | Molecular Weight (kDa): 59.507
UniProt ID(s): C6KT09
PDB ID(s): None
Isoelectric Point: 9.31
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006231 | Malate:quinone-oxidoreductase |
| InterPro | IPR036188 | FAD/NAD(P)-binding domain superfamily |
| PFam | PF06039 | Malate:quinone-oxidoreductase |
| Superfamily | SSF51905 | FAD/NAD(P)-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25843709 | Genetic investigation of tricarboxylic acid metabolism during the Plasmodiumfalciparum life cycle. | Ke H, Lewis IA, ..., Vaidya AB | 10.1016/j.celrep.2015.03.011 |
| 29269266 | Biochemical studies of membrane bound Plasmodium falciparum mitochondrialL-malate:quinone oxidoreductase, a potential drug target. | Hartuti ED, Inaoka DK, ..., Kita K | 10.1016/j.bbabio.2017.12.004 |
| 31234346 | Identification of Plasmodium falciparum Mitochondrial Malate: Quinone Oxidoreductase Inhibitors from the Pathogen Box | Wang X, Miyazaki Y, ..., Kita K | 10.3390/genes10060471 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 34360597 | Biochemical Studies of Mitochondrial Malate: Quinone Oxidoreductase fromToxoplasma gondii | Acharjee R, Talaam KK, ..., Inaoka DK | 10.3390/ijms22157830 |
| 35398098 | Metabolic changes accompanying the loss of fumarate hydratase and malate-quinoneoxidoreductase in the asexual blood stage of Plasmodium falciparum | Rajaram K, Tewari SG, Wallqvist A, Prigge ST | 10.1016/j.jbc.2022.101897 |
| 37036365 | Plasmodium Parasite Malate-Quinone Oxidoreductase Functionally Complements aYeast Deletion Mutant of Mitochondrial Malate Dehydrogenase | Ito T, Kajita S, Fujii M, Shinohara Y | 10.1128/spectrum.00168-23 |
| 28606087 | Suppression of experimental cerebral malaria by disruption of malate:quinoneoxidoreductase | Niikura M, Komatsuya K, ..., Kobayashi F | 10.1186/s12936-017-1898-5 |