Genome location: Pf3D7_06_v3:722,377..731,499(-)
Genome classification: InternalHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Function | GO:0046812 | host cell surface binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.02 | 0.99 |
| Trophozoite | possibly expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | possibly expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0617400
Resistome Missense Mutations: E1946G, I1947F, D1950H, K1953Q, T2204I
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.531 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT15
"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.27) with 5MZA (Global RMSD=2.94)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 1978
Non-coding: 362 | Synonymous: 473 | Nonsynonymous: 1131 | Stop Codon: 12
Protein Length: 2394 | Molecular Weight (kDa): 274.794
UniProt ID(s): A0A191VZ16, A0A191VZ35, A0A191VZD0, A0A5P1A005, A0A5P1A063, A0A5P1A0M8, A0A5P1A0Q1, A0A5P1A0T0, A0A5P1A163, A0A5P1A184, A0A5P1A1B1, A0A5P1A2R9, A0A5P1A2T7, A0A5P1A3D9, A1KQT2, A3R6R9, A3R6S0, C6KT15, P90580, Q26030, Q8T5G0, Q94657, Q95W83
PDB ID(s): None
Isoelectric Point: 6.46
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| InterPro | IPR041480 | Cysteine-rich interdomain region 1 gamma |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| PFam | PF18562 | Cysteine-rich interdomain region 1 gamma |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17562715 | Structural basis for binding of Plasmodium falciparum erythrocyte membraneprotein 1 to chondroitin sulfate and placental tissue and the influence ofprotein polymorphisms on binding specificity. | Beeson JG, Andrews KT, ..., Chai W | 10.1074/jbc.M700231200 |
| 17895392 | PfEMP1-DBL1alpha amino acid motifs in severe disease states of Plasmodiumfalciparum malaria. | Normark J, Nilsson D, ..., Wahlgren M | 10.1073/pnas.0610485104 |
| 18005727 | Plasmodium falciparum erythrocyte membrane protein-1 specifically suppressesearly production of host interferon-gamma. | D'Ombrain MC, Voss TS, ..., Schofield L | 10.1016/j.chom.2007.06.012 |
| 18299339 | The C-terminal segment of the cysteine-rich interdomain of Plasmodium falciparumerythrocyte membrane protein 1 determines CD36 binding and elicits antibodiesthat inhibit adhesion of parasite-infected erythrocytes. | Mo M, Lee HC, ..., Preiser P | 10.1128/IAI.00480-07 |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |
| 19374733 | Structural insights into chondroitin sulphate A binding Duffy-binding-likedomains from Plasmodium falciparum: implications for intervention strategiesagainst placental malaria. | Gill J, Chitnis CE, Sharma A | 10.1186/1475-2875-8-67 |
| 20169064 | Differential recognition of P. falciparum VAR2CSA domains by naturally acquiredantibodies in pregnant women from a malaria endemic area. | Brolin KJ, Persson KE, ..., Chen Q | 10.1371/journal.pone.0009230 |
| 20823248 | Dual stage synthesis and crucial role of cytoadherence-linked asexual gene 9 inthe surface expression of malaria parasite var proteins. | Goel S, Valiyaveettil M, ..., Gowda DC | 10.1073/pnas.1002568107 |
| 21592998 | Positive selection of Plasmodium falciparum parasites with multiple var2csa-typePfEMP1 genes during the course of infection in pregnant women. | Sander AF, Salanti A, ..., Taylor DW | 10.1093/infdis/jir168 |
| 22570492 | Structural and functional insight into how the Plasmodium falciparum VAR2CSAprotein mediates binding to chondroitin sulfate A in placental malaria. | Clausen TM, Christoffersen S, ..., Salanti A | 10.1074/jbc.M112.348839 |
| 23980802 | A molecular switch in the efficiency of translation reinitiation controlsexpression of var2csa, a gene implicated in pregnancy-associated malaria. | Bancells C, Deitsch KW | 10.1111/mmi.12379 |
| 26450557 | Structure of the DBL3X-DBL4epsilon region of the VAR2CSA placental malaria vaccinecandidate: insight into DBL domain interactions. | Gangnard S, Lewit-Bentley A, ..., Gamain B | 10.1038/srep14868 |
| 28351419 | New var reconstruction algorithm exposes high var sequence diversity in a singlegeographic location in Mali. | Dara A, Drabek EF, ..., Silva JC | 10.1186/s13073-017-0422-4 |
| 32471507 | Identification of a conserved var gene in different Plasmodium falciparumstrains. | Dimonte S, Bruske EI, ..., Frank M | 10.1186/s12936-020-03257-x |