Product Description: rhomboid protease ROM10
SignalP Peptide: N/A
# Transmembrane Domains: 7
EC Numbers: 3.4.21.105 (Rhomboid protease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0008233 | peptidase activity |
| Process | GO:0006465 | signal peptide processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | not expressed | 0.08 | 0.95 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | possibly expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0618600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.965 | MFS: -3.036 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1117800)
Modification: Disrupted | RMgm-179
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT26
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.105 | rhomboid protease | dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103838
Most Similar Human Ortholog: C9K011
TM-align score: 0.73 | RMSD: 2.54
Seq Identity: 0.12 | Length: 124 / 274
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000144468 | rhomboid domain containing 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 18 | 5 | 20 |
| disruptive | 6 | 11 | 10 | 30 |
| missense | 6 | 11 | 10 | 29 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 35 | 16 | 15 |
| disruptive | 8 | 38 | 22 | 69 |
| missense | 8 | 35 | 21 | 50 |
PlasmoDB Total SNPs: 136
Non-coding: 119 | Synonymous: 14 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 274 | Molecular Weight (kDa): 31.34
UniProt ID(s): C6KT26
PDB ID(s): None
Isoelectric Point: 8.68
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR022764 | Peptidase S54, rhomboid domain |
| PFam | PF01694 | Peptidase S54, rhomboid domain |
| Superfamily | SSF144091 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 17040128 | Two Plasmodium rhomboid proteases preferentially cleave different adhesinsimplicated in all invasive stages of malaria. | Baker RP, Wijetilaka R, Urban S | 10.1371/journal.ppat.0020113 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 23490234 | Loss-of-function analyses defines vital and redundant functions of the Plasmodiumrhomboid protease family | Lin JW, Meireles P, ..., Khan SM | 10.1111/mmi.12187 |