Product Description: phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1
SignalP Peptide: N/A
# Transmembrane Domains: 6
EC Numbers: 2.4.1.198 (Phosphatidylinositol N-acetylglucosaminyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000506 | glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex |
| Function | GO:0017176 | phosphatidylinositol N-acetylglucosaminyltransferase activity |
| Process | GO:0006506 | GPI anchor biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | nd | nd |
| Trophozoite | no data | nd | nd |
| Schizont | no data | nd | nd |
| Gametocyte | no data | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0618900.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.254 | MFS: -2.777 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1118100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT29
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.1.198 | phosphatidylinositol N-acetylglucosaminyltransferase | iodoacetic acidN-ethylmaleimideSH-alkylating reagentsp-chloromercuriphenylsulfonic acid |
| 2.4.1.198 | phosphatidylinositol N-acetylglucosaminyltransferase | iodoacetic acidN-ethylmaleimideSH-alkylating reagentsp-chloromercuriphenylsulfonic acid |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103823
Most Similar Human Ortholog: B8ZZ29
TM-align score: 0.44 | RMSD: 3.97
Seq Identity: 0.05 | Length: 76 / 729
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000007541 | phosphatidylinositol glycan anchor biosynthesis class Q |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 35 | 26 | 53 |
| disruptive | 10 | 52 | 47 | 127 |
| missense | 10 | 49 | 42 | 109 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 83 | 41 | 64 |
| disruptive | 28 | 170 | 109 | 227 |
| missense | 16 | 140 | 88 | 159 |
PlasmoDB Total SNPs: 250
Non-coding: 201 | Synonymous: 29 | Nonsynonymous: 17 | Stop Codon: 3
Protein Length: 729 | Molecular Weight (kDa): 87.077
PDB ID(s): None
Isoelectric Point: 9.26
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007720 | N-acetylglucosaminyl transferase component |
| PFam | PF05024 | N-acetylglucosaminyl transferase component |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 28459879 | Controlling transferrin receptor trafficking with GPI-valence in bloodstreamstage African trypanosomes | Tiengwe C, Bush PJ, Bangs JD | 10.1371/journal.ppat.1006366 |
| 31401830 | Detection of Anti-Toxoplasma gondii Antibodies in Human Sera Using SyntheticGlycosylphosphatidylinositol Glycans on a Bead-Based Multiplex Assay | Garg M, Stern D, ..., Varon Silva D | 10.1021/acs.analchem.9b02154 |
| 34152830 | Strategic Variants of CSP Delivered as SynDNA Vaccines Demonstrate Heterogeneityof Immunogenicity and Protection from Plasmodium Infection in a Murine Model | Reeder SM, Bah MA, ..., Weiner DB | 10.1128/IAI.00728-20 |