Product Description: acyl-CoA synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0031956 | medium-chain fatty acid-CoA ligase activity |
| Process | GO:0006631 | fatty acid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.34 | 0.84 |
| Trophozoite | expressed | 1.05 | 0.46 |
| Schizont | expressed | 0.22 | 0.87 |
| Gametocyte | not expressed | 0.24 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0619500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.623 | #Insertions: 15
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1118700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1118700)
Modification: Disrupted | RMgm-3029
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT35
"Best" AlphaFill ligand hit: SRT (s,r meso-tartaric acid, Local RMSD=0.28) with 5IE0 (Global RMSD=5.17)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100107
Most Similar Human Ortholog: Q96CM8
TM-align score: 0.71 | RMSD: 3.84
Seq Identity: 0.22 | Length: 501 / 1392
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000167107 | acyl-CoA synthetase family member 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 67 | 35 | 71 |
| disruptive | 32 | 146 | 70 | 216 |
| missense | 26 | 137 | 64 | 200 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 120 | 55 | 95 |
| disruptive | 68 | 305 | 155 | 348 |
| missense | 46 | 264 | 132 | 260 |
PlasmoDB Total SNPs: 140
Non-coding: 51 | Synonymous: 58 | Nonsynonymous: 31 | Stop Codon: 0
Protein Length: 1392 | Molecular Weight (kDa): 163.907
UniProt ID(s): C6KT35
PDB ID(s): None
Isoelectric Point: 7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000399 | TPP-binding enzyme, conserved site |
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR011766 | Thiamine pyrophosphate enzyme, C-terminal TPP-binding |
| InterPro | IPR012001 | Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain |
| InterPro | IPR029035 | DHS-like NAD/FAD-binding domain superfamily |
| InterPro | IPR029061 | Thiamin diphosphate-binding fold |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| PFam | PF02775 | Thiamine pyrophosphate enzyme, C-terminal TPP-binding |
| PFam | PF02776 | Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain |
| Superfamily | SSF52467 | DHS-like NAD/FAD-binding domain superfamily |
| Superfamily | SSF52518 | Thiamin diphosphate-binding fold |
| Superfamily | SSF56801 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 21090257 | [Effect of acetyl-CoA synthase gene overexpression on physiological function ofSaccharomyces cerevisiae] | Chen F, Zhou J, ..., Chen J | https://pubmed.ncbi.nlm.nih.gov/21090257/ |
| 33576609 | Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis | Shi W, Li J, ..., Xiao D | 10.1021/acssynbio.0c00446 |