Genome location: Pf3D7_06_v3:862,450..865,522(+)
Genome classification: Core
Product Description: RNA polymerase subunit sigma apSIG
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0003677 | DNA binding |
| Function | GO:0016987 | sigma factor activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.22 | 0.89 |
| Trophozoite | not expressed | 0.98 | 0.49 |
| Schizont | not expressed | 0.25 | 0.85 |
| Gametocyte | not expressed | 0.57 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0621000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -3.158 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT48
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.04) with 4Q5S (Global RMSD=3.42)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 44 | 31 | 49 |
| disruptive | 10 | 58 | 53 | 132 |
| missense | 9 | 56 | 50 | 107 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 91 | 38 | 51 |
| disruptive | 40 | 166 | 94 | 205 |
| missense | 24 | 133 | 80 | 144 |
PlasmoDB Total SNPs: 143
Non-coding: 70 | Synonymous: 54 | Nonsynonymous: 19 | Stop Codon: 0
Protein Length: 844 | Molecular Weight (kDa): 100.705
UniProt ID(s): C6KT48
PDB ID(s): None
Isoelectric Point: 9.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013324 | RNA polymerase sigma factor, region 3/4-like |
| PFam | N/A | N/A |
| Superfamily | SSF88659 | RNA polymerase sigma factor, region 3/4-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |