Product Description: mRNA-binding protein PUF3
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005730 | nucleolus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0006417 | regulation of translation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.52 | 0.39 |
| Trophozoite | expressed | 0.97 | 0.52 |
| Schizont | expressed | 0.24 | 0.86 |
| Gametocyte | expressed | 0.64 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0621300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.127 | MFS: -3.04 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1121600)
Modification: Tagged | RMgm-4426
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT51
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=8.87) with 3KU2 (Global RMSD=20.18)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102938
Most Similar Human Ortholog: Q15397
TM-align score: 0.67 | RMSD: 4.97
Seq Identity: 0.15 | Length: 538 / 793
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000080608 | pumilio RNA binding family member 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 37 | 17 | 47 |
| disruptive | 36 | 126 | 64 | 140 |
| missense | 21 | 110 | 51 | 117 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 67 | 44 | 52 |
| disruptive | 67 | 263 | 116 | 266 |
| missense | 42 | 204 | 91 | 169 |
PlasmoDB Total SNPs: 252
Non-coding: 148 | Synonymous: 55 | Nonsynonymous: 47 | Stop Codon: 2
Protein Length: 793 | Molecular Weight (kDa): 94.284
UniProt ID(s): C6KT51
PDB ID(s): None
Isoelectric Point: 9.31
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR040059 | Pumilio homologue 3 |
| PFam | N/A | N/A |
| Superfamily | SSF48371 | Armadillo-type fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 29487181 | Puf3 participates in ribosomal biogenesis in malaria parasites | Liang X, Hart KJ, ..., Cui L | 10.1242/jcs.212597 |
| 32317286 | Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 ThroughPhosphorylation of Puf3 in Saccharomyces cerevisiae | Bhondeley M, Liu Z | 10.1534/genetics.120.303191 |
| 38100455 | A non-canonical Puf3p-binding sequence regulates CAT5/COQ7 mRNA under bothfermentable and respiratory conditions in budding yeast | Hayashi S, Iwamoto K, Yoshihisa T | 10.1371/journal.pone.0295659 |
| 37060252 | Yeast Puf3p-mediated mRNA decay is regulated by carbon source-specificdifferential interaction of Puf3p with Pop2p and Yak1p | Bulmash AS, Fischer AD, ..., Olivas WM | 10.1002/1873-3468.14624 |
| 33536101 | Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9and DRBD7 | Erben E, Leiss K, ..., Clayton C | 10.1017/S0031182021000123 |
| 32513341 | Roles of the Pumilio domain protein PUF3 in Trypanosoma brucei growth anddifferentiation | Marucha KK, Clayton C | 10.1017/S003118202000092X |
| 33094674 | Rpb4 and Puf3 imprint and post-transcriptionally control the stability of acommon set of mRNAs in yeast | Garrido-Godino AI, Gupta I, ..., Navarro F | 10.1080/15476286.2020.1839229 |
| 37990816 | MKT1 alleles regulate stress responses through posttranscriptional modulation ofPuf3 targets in budding yeast | Chaithanya KV, Sinha H | 10.1002/yea.3908 |
| 36724944 | Fission Yeast PUF Proteins Puf3 and Puf4 Are Novel Regulators of PI4P5KSignaling | Satoh R, Tanaka T, ..., Sugiura R | 10.1248/bpb.b22-00569 |