Product Description: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.1.1.- (Methyltransferases.);2.8.4.3 (tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0035598 | N6-threonylcarbomyladenosine methylthiotransferase activity |
| Function | GO:0035596 | methylthiotransferase activity |
| Process | GO:0035600 | tRNA methylthiolation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.81 | 0.63 |
| Trophozoite | expressed | 0.70 | 0.62 |
| Schizont | possibly expressed | 0.18 | 0.89 |
| Gametocyte | expressed | 0.59 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0622200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.878 | #Insertions: 4
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1121100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1121100)
Modification: Disrupted | RMgm-3040
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6KT59
"Best" AlphaFill ligand hit: 5AD (5'-deoxyadenosine, Local RMSD=0.56) with 7MJW (Global RMSD=3.69)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101475
Most Similar Human Ortholog: Q5VV42
TM-align score: 0.74 | RMSD: 2.64
Seq Identity: 0.43 | Length: 450 / 860
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000145996 | CDK5 regulatory subunit associated protein 1 like 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 35 | 26 | 54 |
| disruptive | 22 | 86 | 56 | 121 |
| missense | 19 | 70 | 47 | 98 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 82 | 39 | 48 |
| disruptive | 49 | 186 | 94 | 216 |
| missense | 35 | 138 | 77 | 168 |
PlasmoDB Total SNPs: 112
Non-coding: 35 | Synonymous: 51 | Nonsynonymous: 26 | Stop Codon: 0
Protein Length: 860 | Molecular Weight (kDa): 99.459
UniProt ID(s): C6KT59
PDB ID(s): None
Isoelectric Point: 8.68
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005839 | Methylthiotransferase |
| InterPro | IPR006638 | Elp3/MiaB/NifB |
| InterPro | IPR007197 | Radical SAM |
| InterPro | IPR013848 | Methylthiotransferase, N-terminal |
| InterPro | IPR020612 | Methylthiotransferase, conserved site |
| PFam | PF00919 | Methylthiotransferase, N-terminal |
| PFam | PF04055 | Radical SAM |
| Superfamily | SSF102114 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 35164549 | Roles of Ferredoxin-Dependent Proteins in the Apicoplast of Plasmodium falciparumParasites. | Swift RP, Rajaram K, ..., Prigge ST | 10.1128/mbio.03023-21 |