PF3D7_0622500

Genome location: Pf3D7_06_v3:912,197..914,142(-)

Genome classification: Core

Function and Localization

Product Description: RNA methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.193 (16S rRNA (uracil(1498)-N(3))-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008173	RNA methyltransferase activity
Function	GO:0070042	rRNA (uridine-N3-)-methyltransferase activity
Process	GO:0070475	rRNA base methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.05	0.98
Trophozoite	possibly expressed	0.10	0.94
Schizont	possibly expressed	0.04	0.97
Gametocyte	possibly expressed	0.21	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0622500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.979 | MFS: -3.023 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1121400)

Relative Growth Rate: 0.90 ± 0.12
Confidence: 5.56

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1121400)

Modification: Disrupted | RMgm-3042

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT62

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.193	16S rRNA (uracil1498-N3)-methyltransferase	EDTA

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_118254

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	23	6	30
disruptive	7	52	28	87
missense	7	51	27	80

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	48	13	31
disruptive	21	104	57	146
missense	19	95	48	107

PlasmoDB Total SNPs: 31

Non-coding: 23 | Synonymous: 4 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 434 | Molecular Weight (kDa): 50.754

UniProt ID(s): C6KT62

PDB ID(s): None

Isoelectric Point: 9.11

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006700	Ribosomal RNA small subunit methyltransferase E
InterPro	IPR029028	Alpha/beta knot methyltransferases
PFam	PF04452	Ribosomal RNA small subunit methyltransferase E
Superfamily	SSF75217	Alpha/beta knot methyltransferases