PF3D7_0623000 (CS)

Genome location: Pf3D7_06_v3:936,298..938,735(-)

Genome classification: Core

Function and Localization

Product Description: chorismate synthase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.2.3.5 (Chorismate synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Function	GO:0010181	FMN binding
Function	GO:0003959	NADPH dehydrogenase activity
Function	GO:0004107	chorismate synthase activity
Process	GO:0009073	aromatic amino acid family biosynthetic process
Process	GO:0009423	chorismate biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.14	0.93
Trophozoite	no data	0.49	0.71
Schizont	no data	0.15	0.90
Gametocyte	no data	0.19	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0623000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.459 | MFS: -2.7 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1121900)

Relative Growth Rate: 0.85 ± 0.41
Confidence: 3.16

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1121900)

Modification: Disrupted | RMgm-4625

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT66

"Best" AlphaFill ligand hit: FMN (flavin mononucleotide, Local RMSD=0.41) with 1QXO (Global RMSD=5.68)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.3.5	chorismate synthase	O2dioxygenZINC03803450ZINC20149031ZINC13387711ZINC16052528ZINC00602427ZINC01587011ZINC01163259CHEMBL135212CHEMBL338732CHEMBL335859...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P0A2Y6	Chorismate synthase	Streptococcus pneumoniae	OMA, OrthoMCL BLAST	CHEMBL135017CHEMBL132820CHEMBL134761CHEMBL134411...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102633

No human ortholog(s)

Protein Information

Protein Length: 527 | Molecular Weight (kDa): 59.666

UniProt ID(s): C6KT66, O15864

PDB ID(s): None

Isoelectric Point: 7.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000453	Chorismate synthase
InterPro	IPR035904	Chorismate synthase AroC superfamily
PFam	PF01264	Chorismate synthase
Superfamily	SSF103263	Chorismate synthase AroC superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	44	15	34
disruptive	2	45	24	62
missense	2	44	24	55

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	63	25	37
disruptive	12	106	56	111
missense	10	91	47	74

PlasmoDB Total SNPs: 110

Non-coding: 80 | Synonymous: 19 | Nonsynonymous: 11 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
11298276	Subcellular localization and characterization of chorismate synthase in theapicomplexan Plasmodium falciparum.	Fitzpatrick T, Ricken S, ..., Kappes B	10.1046/j.1365-2958.2001.02366.x
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
31381947	Chorismate synthase from malaria parasites is bifunctional enzyme.	Khera HK, Singh SK, Singh S	10.1016/j.molbiopara.2019.111202