PF3D7_0623300

Genome location: Pf3D7_06_v3:947,121..949,262(-)

Genome classification: Core

Function and Localization

Product Description: EGF-like membrane protein, putative

SignalP Peptide: MFRFYQLIIGILLIFYFLEKYNITFC

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.21	0.87
Schizont	expressed	0.05	0.96
Gametocyte	expressed	0.10	0.94

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0623300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.935 | MFS: -2.235 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1122200)

Relative Growth Rate: 0.42 ± 0.14
Confidence: 5.26

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1122200)

Modification: Disrupted | RMgm-3048

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6KT69

"Best" AlphaFill ligand hit: PCF (1,2-diacyl-sn-glycero-3-phoshocholine, Local RMSD=0.11) with 2YGQ (Global RMSD=12.21)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P15514	Amphiregulin	Homo sapiens	OrthoMCL BLAST	CHEMBL347021CHEMBL435462CHEMBL64689CHEMBL363424...

Orthology Information

Ortholog Group (OrthoMCL): OG6_106191

No human ortholog(s)

Protein Information

Protein Length: 236 | Molecular Weight (kDa): 27.419

UniProt ID(s): C6KT69

PDB ID(s): None

Isoelectric Point: 6.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000742	EGF-like domain
PFam	N/A	N/A
Superfamily	SSF57196	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	11	9	12
disruptive	5	7	5	16
missense	5	7	5	15

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	27	5	15
disruptive	8	23	19	36
missense	7	16	18	30

PlasmoDB Total SNPs: 106

Non-coding: 89 | Synonymous: 9 | Nonsynonymous: 8 | Stop Codon: 0